Perfil de esteroides sexuales y cortisol en Pseudoplatystoma metaense x Leiarius marmoratus (Siluriformes: pimelodidae)

Rodríguez-Pulido, José Ariel; Nieto-Vera, Mónica T.; Villalba Rey, Deicy; Moreno-Guerra, Yeferson A.; Mira-López, Tatiana; Cruz-Casallas, Pablo

Publicación:
Perfil de esteroides sexuales y cortisol en Pseudoplatystoma metaense x Leiarius marmoratus (Siluriformes: pimelodidae)

dc.contributor.author	Rodríguez-Pulido, José Ariel
dc.contributor.author	Nieto-Vera, Mónica T.
dc.contributor.author	Villalba Rey, Deicy
dc.contributor.author	Moreno-Guerra, Yeferson A.
dc.contributor.author	Mira-López, Tatiana
dc.contributor.author	Cruz-Casallas, Pablo
dc.date.accessioned	2024-12-03T20:07:34Z
dc.date.available	2024-12-03T20:07:34Z
dc.date.issued	2024
dc.description	Incluye gráficas.	spa
dc.description.abstract	Desde hace décadas se ha determinado que los esteroides desempeñan un papel importante en la coordinación de las actividades del eje hipotálamo-hipófisis-gónada, además de su actividad en el control de múltiples procesos incluidos el apareamiento y reproducción. Con el fin de evidenciar la funcionalidad de las gónadas de híbridos de Rayaque (Pseudoplatystoma metaense x Leiarius marmoratus), fueron medidos los niveles plasmáticos de testosterona (T), estradiol (E2) y cortisol, utilizando los kits comerciales Acculite CLIA microwells (Monobind Inc. ®) Testosterone Kit System 3775-300, estradiol Kit System 4975-300 y cortisol Kit System 3675-300. La lectura final se llevó a cabo en el equipo LumiStat 4100 Plus de Awareness Technology, Inc. ®. Se extrajo una muestra de sangre a cada individuo (n=10) que fue procesada y analizada cada 40-45 días y durante 8 muestreos. Los niveles plasmáticos de T fueron más bajos en las hembras (0,01 a 0,35 ng/ml) y en los machos se registraron niveles altos de junio a julio (0,65 ng/ml), relacionados. Con los periodos de mayor pluviosidad en la región que desencadena el inicio de la temporada reproductiva. Los niveles de E2 fueron mayores en las hembras (0,88 ng/ml), posiblemente asociados con la maduración ovárica y actividad de la aromatasa. En tanto que los machos muestran descensos (0,1 ng/ml) para junio- julio, con un incremento posterior alcanzando un valor máximo de 0,7 ng/ml. Los niveles de cortisol se incrementaron hacia la temporada de reproducción, siendo siempre mayores en hembras (12,9 a 34,5 ng/ml).	spa
dc.description.abstract	For decades it has been determined that steroids play an important role in coordinating the activities of the hypothalamic-pituitary-gonad axis, in addition to their activity in the control of multiple processes including mating and reproduction. In order to demonstrate the functionality of the gonads of Rayaque hybrids (Pseudoplatystoma metaense x Leiarius marmoratus), plasma levels of testosterone (T), estradiol (E2) and cortisol were measured, using commercial kits Acculite CLIA microwells (Monobind Inc. ®) Testosterone Kit System 3775-300, estradiol Kit System 4975-300 and cortisol Kit System 3675-300. The final reading was carried out on the LumiStat 4100 Plus device from Awareness Technology, Inc. ®. A blood sample was taken from each individual (n = 10) that was processed and analyzed every 40-45 days and for 8 samples. Plasma T levels were lower in females (0.01 to 0.35 ng / ml) and in males there were high levels from June to July (0.65 ng / ml), related. With periods of greater rainfall in the region that triggers the beginning of the reproductive season. E2 levels were higher in females (0.88 ng / ml), possibly associated with ovarian maturation and aromatase activity. While the males show decreases (0.1 ng / ml) for June-July, with a subsequent increase reaching a maximum value of 0.7 ng / ml. Cortisol levels increased towards the breeding season, being always higher in females (12.9 to 34.5 ng / ml).	eng
dc.format.extent	19 páginas
dc.format.mimetype	application/pdf
dc.identifier.citation	Rodríguez-Pulido, José A., Nieto-Vera, Mónica T., Villalba Rey, D., Moreno-Guerra, Yeferson A., Mira-López, T. y Cruz-Casallas, P. (2021). Perfil de esteroides sexuales y cortisol en Pseudoplatystoma metaense x Leiarius marmoratus (Siluriformes: pimelodidae) [Documento de trabajo] Repositorio digital Universidad de los Llanos.
dc.identifier.instname	Universidad de los Llanos
dc.identifier.reponame	Repositorio digital Universidad de los Llanos
dc.identifier.repourl	https://repositorio.unillanos.edu.co
dc.identifier.uri	https://repositorio.unillanos.edu.co/handle/001/4921
dc.language.iso	spa
dc.publisher	Universidad de los Llanos
dc.publisher.place	Villavicencio, Meta
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dc.rights	Derechos Reservados - Universidad de los Llanos, 2024	spa
dc.rights.accessrights	info:eu-repo/semantics/openAccess
dc.rights.coar	http://purl.org/coar/access_right/c_abf2
dc.rights.license	Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)
dc.rights.uri	https://creativecommons.org/licenses/by-nd/4.0/
dc.subject.proposal	Perfil hormonal	spa
dc.subject.proposal	Testosterona	spa
dc.subject.proposal	Estradiol	spa
dc.subject.proposal	CLIA	spa
dc.subject.proposal	Híbridos de peces	spa
dc.subject.proposal	Hormonal profile	eng
dc.subject.proposal	Testosterone	eng
dc.subject.proposal	Estradiol	eng
dc.subject.proposal	Fish hybrids	eng
dc.title	Perfil de esteroides sexuales y cortisol en Pseudoplatystoma metaense x Leiarius marmoratus (Siluriformes: pimelodidae)	spa
dc.title	Profile of sexual steroids and cortisol in Pseudoplatystoma metaense x Leiarius marmoratus (Siluriformes: pimelodidae)	eng
dc.type	Documento de trabajo
dc.type.category	Divulgación pública de la ciencia
dc.type.coar	http://purl.org/coar/resource_type/c_8042
dc.type.coarversion	http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.content	Text
dc.type.driver	info:eu-repo/semantics/workingPaper
dc.type.version	info:eu-repo/semantics/publishedVersion
dspace.entity.type	Publication