Hepatic transcriptomic response in aequidens metae to Polycyclic Aromatic Hydrocarbons (PAH)

Corredor-Santamaría, Wilson; Mulet, Maite de María; Pinzón-Velasco, Andrés M.; Arbel, Ziv; Velasco Santamaría, Yohana María

Publicación:
Hepatic transcriptomic response in aequidens metae to Polycyclic Aromatic Hydrocarbons (PAH)

dc.contributor.author	Corredor-Santamaría, Wilson
dc.contributor.author	Mulet, Maite de María
dc.contributor.author	Pinzón-Velasco, Andrés M.
dc.contributor.author	Arbel, Ziv
dc.contributor.author	Velasco Santamaría, Yohana María
dc.date.accessioned	2024-12-04T23:53:29Z
dc.date.available	2024-12-04T23:53:29Z
dc.date.issued	2021-09-29
dc.description.abstract	Aquatic systems receive Polycyclic aromatic hydrocarbons (PAH) through atmospheric deposition and direct runoff release, being fish one of the targets with a particular interest because they occupy key positions in aquatic and terrestrial food webs. In fact, exposure to different PAH can generate specific responses in gene expression profiles associated with biotransformation that could be used as biomarkers of exposure to these hydrocarbons. In this regard, RNA sequencing (RNA-seq) has provided insight into response mechanisms in gene expression studies by integrating data on biological effects in response to sublethal PAH exposure in non-model organisms without available genomic information. However, there are no transcriptomic profiling studies in fish in Colombia. Therefore, the objective of the present study was to characterize the hepatic transcriptional response in the non-model fish Aequidens metae exposed to polycyclic aromatic hydrocarbons under acute exposure.Liver samples were taken from adult specimens exposed via intraperitoneal to six conditions with five replicates, no injection control, solvent vehicle control, ßnaphthoflavone (BNF), naphthalene (NAP), phenanthrene (PHE) and benzo[a]pyrene (BaP), transcriptomic analysis of RNA-seq derived data was performed. A total of 396 differentially expressed genes (DEG) were identified for the comparison between solvent and BNF, 330 for NAP, 396 for PHE and 381 for BaP of which 15 genes were common to all 4 PAH. In turn, enrichment analysis identified 9, 31, 12 and 31 KEGG pathways for BNF, NAP, PHE and BaP, respectively. In general, the pathways affected were associated with the processes of cell growth and death; lipid and carbohydrate metabolism; transport and catabolism; folding, sorting and degradation; the endocrine system; signal transduction; xenobiotic metabolism; nucleotide metabolism; viral infectious diseases and the endocrine system. Overall, the analysis showed evidence of toxic effects on liver tissue in fish exposed to the different PAH underlying molecular mechanisms through the study of transcriptomic profiling being a pioneer study in Colombian fish in this area. Acknowledgment: This study was funded by Colciencias-ANH and Universidad de los Llanos project No 162-2016 and also a Project from the Pontificia Universidad Javeriana from Colombia.	eng
dc.format.extent	130 páginas
dc.format.mimetype	application/pdf
dc.identifier.citation	Corredor-Santamaría, W., Mulet, Maite de María., Pinzón-Velasco, Andrés M., Arbel, Ziv y Velasco Santamaría, Yohana M. (2021). Hepatic transcriptomic response in aequidens metae to Polycyclic Aromatic Hydrocarbons (PAH). Repositorio digital Universidad de los Llanos.
dc.identifier.instname	Universidad de los Llanos
dc.identifier.reponame	Repositorio digital Universidad de los Llanos
dc.identifier.repourl	https://repositorio.unillanos.edu.co
dc.identifier.uri	https://repositorio.unillanos.edu.co/handle/001/4929
dc.language.iso	eng
dc.publisher	Society of Environmental Toxicology and Chemistry (SETAC) Latin America 14 Biennial Meeting
dc.publisher.place	Villavicencio, Meta
dc.rights	Derechos Reservados - Universidad de los Llanos, 2021	spa
dc.rights.accessrights	info:eu-repo/semantics/openAccess
dc.rights.coar	http://purl.org/coar/access_right/c_abf2
dc.rights.license	Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.source	https://www.setac.org/resource/setac-la-virtual-2021-abstract-book.html
dc.subject.proposal	RNA-seq	eng
dc.subject.proposal	Aequidens	eng
dc.subject.proposal	PAH	eng
dc.subject.proposal	Xenobiotic metabolism	eng
dc.title	Hepatic transcriptomic response in aequidens metae to Polycyclic Aromatic Hydrocarbons (PAH)	spa
dc.type	Evento científico
dc.type.category	Divulgación pública de la ciencia
dc.type.coar	http://purl.org/coar/resource_type/c_8042
dc.type.coarversion	http://purl.org/coar/version/c_970fb48d4fbd8a85
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dc.type.driver	info:eu-repo/semantics/workingPaper
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