Induction of cytochrome P4501A and biliary metabolites of pyrene and phenanthrene in Aequidens metae under laboratory conditions

Mora-Solarte, Diego Alejandro; Calderón-Delgado, Ivonne; Velasco Santamaría, Yohana María

Publicación:
Induction of cytochrome P4501A and biliary metabolites of pyrene and phenanthrene in Aequidens metae under laboratory conditions

dc.contributor.author	Mora-Solarte, Diego Alejandro
dc.contributor.author	Calderón-Delgado, Ivonne
dc.contributor.author	Velasco Santamaría, Yohana María
dc.date.accessioned	2024-11-15T22:19:43Z
dc.date.available	2024-11-15T22:19:43Z
dc.date.issued	2018-09-18
dc.description.abstract	Oil toxicity is a subject of ongoing research and it is important to gain knowledge about baseline levels in order to evaluate the environmental risk assessments associate to oil pollution in aquatic environments and to determine possible adverse effects on aquatic organisms. The study of biomarkers is suggested as an appropriate strategy to study the sublethal effects of contaminants, providing an early indication of possible adverse effects on organisms. The aim of this study was to evaluated two biomarkers in a freshwater fish commonly known as luminosa (Aequidens metae) through acute exposure to pyrene and phenanthrene by intraperitoneal induction. The fish were exposed for 10 days to nominal concentrations of 1, 10 and 50 ppm of pyrene and phenanthrene. Fish were sampled three times during the exposure time. In each fish, liver and bile were collected and kept in -80 ºC until the analysis. The induction of cytochrome P450 in the liver was estimated by ethoxyresorufin-Odeethylase (EROD) activity and the determination of PAH metabolites in bile was measured. The hepatic EROD activity showed an increase and biliary metabolites of pyrene and phenanthrene showed decreasing levels after the first sampling time, indicating the biotransformation efficiency of PAHs. Fish exposed to the highest concentration of pyrene showed higher levels of pyrene metabolites in bile and higher CYP1A induction evidencing monotonic effect. These dose-response data can be used as useful contributions in assessing environmental risk with respect to effluent pollution from the oil industry in Colombia.	eng
dc.description.notes	Latin America 13th Biennial Meeting. 15-18 septiembre 2919, Cartagena, Colombia.	spa
dc.format.extent	140 páginas
dc.format.mimetype	application/pdf
dc.identifier.citation	Mora Solarte, D. A., Calderón Delgado, I. y Velasco Santamaría, Y. M. (2019). Induction of cytochrome P4501A and biliary metabolites of pyrene and phenanthrene in Aequidens metae under laboratory conditions (p. 84). Universidad de los Llanos.
dc.identifier.instname	Universidad de los Llanos
dc.identifier.reponame	Repositorio digital Universidad de los Llanos
dc.identifier.repourl	https://repositorio.unillanos.edu.co
dc.identifier.uri	https://repositorio.unillanos.edu.co/handle/001/4827
dc.language.iso	eng
dc.publisher	Society of Environmental Toxicology and Chemistry (SETAC) Latin America 13 Biennial Meeting
dc.publisher.place	Cartagena, Colombia
dc.rights	Derechos Reservados - Universidad de los Llanos, 2018	spa
dc.rights.accessrights	info:eu-repo/semantics/openAccess
dc.rights.coar	http://purl.org/coar/access_right/c_abf2
dc.rights.license	Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)
dc.rights.uri	https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subject.proposal	Oil pollution	eng
dc.subject.proposal	Pyrene	eng
dc.subject.proposal	Phenanthrene	eng
dc.subject.proposal	Biliary metabolites	eng
dc.subject.proposal	EROD	eng
dc.title	Induction of cytochrome P4501A and biliary metabolites of pyrene and phenanthrene in Aequidens metae under laboratory conditions	eng
dc.type	Evento científico
dc.type.category	Divulgación pública de la ciencia
dc.type.coar	http://purl.org/coar/resource_type/c_8544
dc.type.coarversion	http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.content	Text
dc.type.driver	info:eu-repo/semantics/conferenceObject
dc.type.version	info:eu-repo/semantics/publishedVersion
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person.identifier.gsid	https://scholar.google.com/citations?user=NX49diwAAAAJ&hl=en
person.identifier.orcid	0000-0002-6845-1663
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