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Obtención de péptidos con posible bioactividad a partir de lodos digeridos derivados de la biodigestión anaerobia de aguas residuales

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dc.contributor.advisorSegura Latorre, Cesar
dc.contributor.advisorPachón García, Jorge
dc.contributor.authorBarreto Montenegro, Julian Camilo
dc.date.accessioned2023-09-26T21:17:38Z
dc.date.available2023-09-26T21:17:38Z
dc.date.issued2016
dc.descriptionFiguras y tablasspa
dc.description.abstractLa digestión anaerobia es un proceso fermentativo en ausencia de oxígeno llevado a cabo por comunidades microbianas. Se ha utilizado como una alternativa para el manejo de diversas fuentes residuales y para la producción de biogás. Entender las complejas interacciones y los procesos que involucran a estas comunidades microbianas es crítico para el mejoramiento de las condiciones anaerobias y buscar nuevas aplicaciones. Objetivo: Diseñar una metodología para la extracción y caracterización de péptidos derivados de la digestión anaerobia de lodos de la planta de tratamiento de aguas residuales de San Fernando (Itagüí, Antioquia). Para lograr este objetivo, el material peptídico se extrajo a partir de los lodos y se determinó la actividad antimicrobiana del extracto proteico. Metodología: Los péptidos se extrajeron mecánicamente por sonicación y se tamizaron a través de filtros moleculares de 10 KDa de peso molecular. La presencia y concentración de material proteico en los extractos (péptidos/proteínas pequeñas) se determinó por el método de Lowry. El extracto se caracterizó por cromatografía líquida de alta eficacia (HPLC), acoplado a espectrometría de masas de baja resolución (LC-MS) y electroforesis en gel (Tricina-SDS-PAGE).spa
dc.description.abstractAnaerobic digestion is a fermentative proccess in the absence of oxygen. Carried out by microbial comunities, it has been an alternative for management of diverse sources of waste and biogas generation. Understanding the complex interactions and procesess involving microbial communities is critical for the improvement of anaerobic conditions and looking for new applications. Aim: Design a methodology for extraction and charaterization of peptides generated by anaerobic digestion of sludges from the Planta de Tratamiento de Aguas Residuales de San Fernando (Itagüí, Antioquía). To get this aim, peptidic material from sludge were extracted and the antimicrobial activity of the proteic extract was determined. Methodology: Peptides were mechanically extracted by sonication and filtered throughout 10 kDa molecular weight cutoff filters. Presence and concentration of proteic material in the extracts (peptides/small proteins) was determined by Lowry method, The extract was characterized by High Performance Liquid Chromatography (HPLC) online to low resolution mass spectrometry (LC-MS) and Tricine-SDSPAGE gel electrophoresis.eng
dc.description.degreelevelPregradospa
dc.description.degreenameBiólogospa
dc.description.programBiologíaspa
dc.description.tableofcontentsPlanteamiento del problema. -- Hipótesis. -- Objetivos. -- Objetivo general. -- Objetivos específicos. -- Justificación. -- Marco teórico. -- Digestión anaerobia. – Péptidos. – Metodología. -- Recolección de la muestra. -- Extracción de péptidos. -- Determinación de la concentración de proteína péptidos. -- Prueba de actividad antimicrobiana. -- Electroforesis Tricina-SDS-PAGE. -- Caracterización preliminar por HPLC. -- Cromatografía liquida acoplada a espectrometría de masa (LC-MS). -- Resultados. -- Determinación de la presencia de péptidos en el extracto. -- Prueba de actividad antimicrobiana. -- Electroforesis Tricina-SDS-PAGE. -- Caracterización preliminar por HPLC. -- Análisis por espectrometría de masas. -- Discusión. -- Determinación de la presencia/concentración de péptidos en el extracto. -- Prueba de actividad antimicrobiana. -- Electroforesis Tricina-SDS-PAGE. -- Caracterización preliminar por HPLC. -- Análisis por espectrometría de masas. – Conclusiones. -- Dificultades. -- Sugerencias. – Bibliografía. -- Anexos.spa
dc.format.extent136 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.citationBarreto Montenegro, J. (2016). Obtención de péptidos con posible bioactividad a partir de lodos digeridos derivados de la biodigestión anaerobia de aguas residuales [Trabajo de grado, Universidad de los Llanos]. Repositorio digital Universidad de los Llanos. https://repositorio.unillanos.edu.co/handle/001/3024spa
dc.identifier.instnameUniversidad de los Llanosspa
dc.identifier.reponameRepositorio digital Universidad de los Llanosspa
dc.identifier.repourlhttps://repositorio.unillanos.edu.co/spa
dc.identifier.urihttps://repositorio.unillanos.edu.co/handle/001/3024
dc.language.isospaspa
dc.publisherUniversidad de los Llanosspa
dc.publisher.facultyFacultad de Ciencias Básicas e Ingenieríaspa
dc.publisher.placeVillavicenciospa
dc.relation.indexedN/Aspa
dc.relation.referencesAbram, F., Enright, A. M., O´Reilly, J., Botting, C. H., Collins, G., & O´Flaherty, V. (2011). A metaproteomic approach gives functional insights into anaerobic digestion. Journal of Applied Microbiology, 110, 1550–1560.spa
dc.relation.referencesAbram, F., Gunnigle, E., & O’Flaherty, V. (2009). Optimisation of protein extraction and 2-DE for metaproteomics of microbial communities from anaerobic wastewater treatment biofilms. Electrophoresis, 30, 4149–4151.spa
dc.relation.referencesAcosta, Y., Cristina, M., & Abreu, O. (2005). La digestión anaerobia. Aspectos teóricos. Parte I. ICIDCA. Sobre los Derivados de la Caña de Azúcar, XXXlX(1), 35–48.spa
dc.relation.referencesAguilar, M. (2004). HPLC of Peptides and Proteins. Methods and Protocols. (M. Aguilar, Ed.), Methods in Molecular Biology (Vol. 251). New Jersey: Humana Press.spa
dc.relation.referencesAguilar, M.-I. (2003). Reversed-Phase High-Performance Liquid Chromatography. En M. Aguilar (Ed.), HPLC of Peptides and Proteins. Methods and Protocols (Vol. 251, pp. 9–22). New Jersey: Humana Press.spa
dc.relation.referencesAli Shah, F., Mahmood, Q., Maroof Shah, M., Pervez, A., & Ahmad Asad, S. (2014). Microbial ecology of anaerobic digesters: The key players of anaerobiosis. The Scientific World Journal, 183752, 1–21.spa
dc.relation.referencesAmani, T., Nosrati, M., & Sreekrishnan, T. R. (2010). Anaerobic digestion from the viewpoint of microbiological, chemical, and operational aspects — a review. Environmental Reviews, 18, 255–278.spa
dc.relation.referencesBals, R. (2000). Epithelial antimicrobial peptides in host defense against infection. Respiratory research, 1(3), 141–50.spa
dc.relation.referencesBatdorj, B., Dalgalarrondo, M., Choiset, Y., Pedroche, J., Métro, F., Prévost, H., Chobert, J. M.spa
dc.relation.referencesHaertlé, T. (2006). Purification and characterization of two bacteriocins produced by lactic acid bacteria isolated from Mongolian airag. Journal of Applied Microbiology, 101(December 2005), 837–848.spa
dc.relation.referencesBedoya-Urrego, K., Acevedo-Ruíz, J. M., Peláez-Jaramillo, C. A., & Agudelo-López, S. del P. (2013). Caracterización de biosólidos generados en la planta de tratamiento de agua residual San Fernando, Itagüí (Antioquia, Colombia). Revista de Salud Pública, 15(5), 778–790.spa
dc.relation.referencesBiosa, G., Addis, M. F., Tanca, A., Pisanu, S., Roggio, T., Uzzau, S., & Pagnozzi, D. (2011). Comparison of blood serum peptide enrichment methods by Tricine SDS-PAGE and mass spectrometry. Journal of Proteomics, 75(1), 93–99.spa
dc.relation.referencesBranco, P., Francisco, D., Chambon, C., Hébraud, M., Arneborg, N., Almeida, M. G., Caldeira, J., & Albergaria, H. (2014). Identification of novel GAPDH-derived antimicrobial peptides secreted by Saccharomyces cerevisiae and involved in wine microbial interactions. Applied Microbiology and Biotechnology, 98, 843–853.spa
dc.relation.referencesBrogden, K. A. (2005). Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria? Nature reviews. Microbiology, 3(3), 238–50.spa
dc.relation.referencesCao, Y., Chang, Z.-Z., Ma, Y., Yang, H., & Fu, G.-Q. (2013). Control mechanism of phytophthora blight of chilli pepper by anaerobically digested pig slurry—The roles of ammonium and humic acid in biogas slurry. Chinese Journal of Eco-Agriculture, 9, 0–14.spa
dc.relation.referencesChong, G., Kimyon, O., Rice, S. A., Kjelleberg, S., & Manefield, M. (2012). The presence and role of bacterial quorum sensing in activated sludge. Microbial biotechnology, 5(5), 621–33.spa
dc.relation.referencesClarkson, J., Cui, Z., & Darton, R. (2000). Effect of solution conditions on protein damage in foam. Biochemical Engineering Journal, 4(2), 107–114.spa
dc.relation.referencesCotter, P. D., Hill, C., & Ross, R. P. (2005). Bacteriocins: developing innate immunity for food. Nature reviews. Microbiology, 3(10), 777–88.spa
dc.relation.referencesCowan, M. M. (1999). Plant Products as Antimicrobial Agents. Clinical Microbiology Reviews, 12(4), 564–582.spa
dc.relation.referencesCui, Q., Lewis, I. A., Hegeman, A. D., Anderson, M. E., Li, J., Schulte, C. F., Westler, W. M., Eghbalnia, H. R., Sussman, M. R., & Markley, J. L. (2008). Metabolite identification via the Madison Metabolomics Consortium Database. Nature biotechnology, 26(2), 162–4.spa
dc.relation.referencesDong, H., Kemptner, J., Marchetti-Deschmann, M., Kubicek, C. P., & Allmaier, G. (2009). Development of a MALDI two-layer volume sample preparation technique for analysis of colored conidia spores of Fusarium by MALDI linear TOF mass spectrometry. Analytical and bioanalytical chemistry, 395(5), 1373–83.spa
dc.relation.referencesDong, X., Xin, Y., Jian, W., Liu, X., & Ling, D. (2000). Bifidobacterium thermacidophilum sp. nov., isolated from an anaerobic digester. International journal of systematic and evolutionary microbiology, 50 Pt 1, 119–25.spa
dc.relation.referencesDride, D., & Rebuffat, S. (2011). Prokaryotic antimicrobial peptides: from genes to applications. (S. Rebuffat & D. Dride, Eds.), Media. New York: Springer.spa
dc.relation.referencesEmpresas Públicas de Medellín. (s. f.). Tratamiento de aguas residuales. Planta San Fernando.spa
dc.relation.referencesFeng, H., Qu, G. F., Ning, P., Xiong, X. F., Jia, L. J., Shi, Y. K., & Zhang, J. (2011). The resource utilization of anaerobic fermentation residue. Procedia Environmental Sciences, 11(PART C), 1092–1099.spa
dc.relation.referencesFountoulakis, M., Drillia, P., Stamatelatou, K., & Lyberatos, G. (2004). Toxic effect of pharmaceuticals on methanogenesis. Water Science and Technology, 50(5), 335–340.spa
dc.relation.referencesGarcia, S. L., Jangid, K., Whitman, W. B., & Das, K. C. (2011). Transition of microbial communities during the adaption to anaerobic digestion of carrot waste. Bioresource Technology, 102(15), 7249–7256.spa
dc.relation.referencesHammami, R., Zouhir, A., Le Lay, C., Ben Hamida, J., & Fliss, I. (2010). BACTIBASE second release: a database and tool platform for bacteriocin characterization. BMC microbiology, 10(1), 22.spa
dc.relation.referencesHanreich, A., Heyer, R., Benndorf, D., Rapp, E., Pioch, M., Reichl, U., & Klocke, M. (2012). Metaproteome analysis to determine the metabolically active part of a thermophilic microbial community producing biogas from agricultural biomass. Canadian Journal of Microbiology, 58(7), 917–922.spa
dc.relation.referencesHassan, M., Kjos, M., Nes, I. F., Diep, D. B., & Lotfipour, F. (2012). Natural antimicrobial peptides from bacteria: Characteristics and potential applications to fight against antibiotic resistance. Journal of Applied Microbiology, 113, 723–736.spa
dc.relation.referencesHayes, M., Stanton, C., Fitzgerald, G. F., & Ross, R. P. (2007). Putting microbes to work: Diary fermentation, cell factories and bioactive peptides. Part II: Bioactive peptide functions. Biotechnology Journal, 2, 435–449.spa
dc.relation.referencesHegemann, J. D., Zimmermann, M., Xie, X., & Marahiel, M. A. (2015). Lasso Peptides: An Intriguing Class of Bacterial Natural Products. Accounts of Chemical Research, 48(7), 1909–1919.spa
dc.relation.referencesHeyer, R., Kohrs, F., Benndorf, D., Rapp, E., Kausmann, R., Heiermann, M., Klocke, M., & Reichl, U. (2013). Metaproteome analysis of the microbial communities in agricultural biogas plants. New Biotechnology, 30(6), 614–622.spa
dc.relation.referencesHorai, H., Arita, M., Kanaya, S., Nihei, Y., Ikeda, T., Suwa, K., … Nishioka, T. (2010). MassBank: a public repository for sharing mass spectral data for life sciences. Journal of mass spectrometry : JMS, 45(7), 703–14.spa
dc.relation.referencesJack, R., Tagg, J., & Ray, B. (1995). Bacteriocins of gram-positive bacteria. Microbiol. Rev., 59(2), 171–200.spa
dc.relation.referencesJudd, R. C. (1994). Electrophoresis of peptides. En J. M. Walker (Ed.), Methods in molecular biology (Clifton, N.J.) (Vol. 32, pp. 49–57). Huma Press Inc.spa
dc.relation.referencesKawashima, Y., Fukutomi, T., Tomonaga, T., Takahashi, H., Nomura, F., Maeda, T., & Kodera, Y. (2010). High-yield peptide-extraction method for the discovery of subnanomolar biomarkers from small serum samples. Journal of Proteome Research, 9(4), 1694–1705.spa
dc.relation.referencesKleerebezem, M., Quadri, L. E. N., Kuipers, O. P., & de Vos, W. M. (1997). Quorum sensing by peptide pheromones and two-component signal-transduction systems in Gram-positive bacteria. Molecular Microbiology, 24(5), 895–904.spa
dc.relation.referencesKrause, L., Diaz, N. N., Edwards, R. A., Gartemann, K.-H., Krömeke, H., Neuweger, H., … Goesmann, A. (2008). Taxonomic composition and gene content of a methane-producing microbial community isolated from a biogas reactor. Journal of biotechnology, 136(1-2), 91–101.spa
dc.relation.referencesKuhn, R., Benndorf, D., Rapp, E., Reichl, U., Palese, L. L., & Pollice, A. (2011). Metaproteome analysis of sewage sludge from membrane bioreactors. Proteomics, 11(13), 2738–2744.spa
dc.relation.referencesKümmerer, K. (2009). Antibiotics in the aquatic environment--a review--part I. Chemosphere, 75(4), 417–34.spa
dc.relation.referencesLamendella, R., Santo Domingo, J. W., Kelty, C., & Oerther, D. B. (2008). Bifidobacteria in feces and environmental waters. Applied and environmental microbiology, 74(3), 575–84.spa
dc.relation.referencesLaukova, a., Marekova, M., Vasilkova, Z., Papojava, I., & Juris, P. (2002). Selected microbial consortium of row and digested slurry and its susceptibility to enterocins. Word Journal of Microbiology and Biotechnology, 18, 11–15.spa
dc.relation.referencesLewis, K. (2013). Platforms for antibiotic discovery. Nature reviews. Drug discovery, 12(5), 371– 87.spa
dc.relation.referencesLiu, X., Inbar, Y., Dorrestein, P. C., Wynne, C., Edwards, N., Souda, P., Whitelegge, J. P., Bafna, V., & Pevzner, P. A. (2010). Deconvolution and database search of complex tandem mass spectra of intact proteins: a combinatorial approach. Molecular & cellular proteomics : MCP, 9(12), 2772–82.spa
dc.relation.referencesLiu, X., Sirotkin, Y., Shen, Y., Anderson, G., Tsai, Y. S., Ting, Y. S., Goodlett, D. R., Smith, R. D., Bafna, V., & Pevzner, P. A. (2012). Protein Identification Using Top-Down. Molecular & Cellular Proteomics, 11(6), M111.008524–M111.008524.spa
dc.relation.referencesLoans, I., Run, R., Library, S., Lu, S., Walters, G., Parg, R., & Dutcher, J. R. (2014). Nanomechanical response of bacterial cells to cationic antimicrobial peptides. Soft Matter, 10, 1806–1815.spa
dc.relation.referencesLowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. The Journal of Biological Chemestry, 193(1), 265–275.spa
dc.relation.referencesMa, Y., Li, H., Chang, Z.-Z., Xu, Y., & Zhang, J. (2011). Biocontrol Effect and Mechanism of Biogas Slurry on Plant Disease Ⅰ:Primary Study of Growth Inhibition Effects and Mechanism on Phytopathogen Fung. Chinese Journal of Eco-Agriculture, 2.spa
dc.relation.referencesManes, N. P., Gustin, J. K., Rue, J., Mottaz, H. M., Purvine, S. O., Norbeck, A. D., Monroe, M. E., Zimmer, J. S. D., Metz, T. O., Adkins, J. N., Smith, Richard, D., & Heffron, F. (2007). Targeted protein degradation by Salmonella under phagosome-mimicking culture conditions investigated using comparative peptidomics. Molecular & cellular proteomics : MCP, 6(4), 717–27.spa
dc.relation.referencesManyi-Loh, C. E., Mamphweli, S. N., Meyer, E. L., Okoh, A. I., Makaka, G., & Simon, M. (2014). Inactivation of selected bacterial pathogens in dairy cattle manure by mesophilic anaerobic digestion (balloon type digester). International Journal of Environmental Research and Public Health, 11(7), 7184–94.spa
dc.relation.referencesMarques, S. S. I., Nascimento, I. A., De Almeida, P. F., & Chinalia, F. A. (2013). Growth of Chlorella vulgaris on sugarcane vinasse: The effect of anaerobic digestion pretreatment. Applied Biochemistry and Biotechnology, 171, 1933–1943.spa
dc.relation.referencesMartín, J., Camacho-Muñoz, D., Santos, J. L., Aparicio, I., & Alonso, E. (2012). Occurrence of pharmaceutical compounds in wastewater and sludge from wastewater treatment plants: removal and ecotoxicological impact of wastewater discharges and sludge disposal. Journal of hazardous materials, 239-240, 40–47.spa
dc.relation.referencesMcNaught, A. D. (1984). Nomenclature and Symbolism for Amino Acids and Peptides. Recommendations 1983. European Journal of Biochemistry, 138(1), 9–37.spa
dc.relation.referencesMiao, X. S., Bishay, F., Chen, M., & Metcalfe, C. D. (2004). Occurrence of antimicrobials in the final effluents of wastewater treatment plants in Canada. Environmental Science and Technology, 38(13), 3533–3541.spa
dc.relation.referencesMo, Y. (1998). Antimicrobial test of biogas fluid on plant pathogenic microorganism. China Biogas, 6(2), 6–10.spa
dc.relation.referencesMurillo, W., Araque, P., & Peláez, C. A. (2012). Actividad fungicida e insecticida de emulsiones agua/aceite de mezclas de extractos de Nicotiana tabacum, Azadiractha indica y Eucalyptus tereticornis. Informacion Tecnologica, 23(1), 139–152.spa
dc.relation.referencesNissen-Meyer, J., & Nes, I. F. (1997). Ribosomally synthesized antimicrobial peptides: their function, structure, biogenesis, and mechanism of action. Archives of Microbiology, 167(23), 67–77.spa
dc.relation.referencesPark, C., & Helm, R. F. (2008). Application of metaproteomic analysis for studying extracellular polymeric substances (EPS) in activated sludge flocs and their fate in sludge digestion. Water Science and Technology, 57(12), 2009–2015.spa
dc.relation.referencesPerez-Riverol, Y., Wang, R., Hermjakob, H., Müller, M., Vesada, V., & Vizcaíno, J. A. (2014). Open source libraries and frameworks for mass spectrometry based proteomics: a developer’s perspective. Biochimica et biophysica acta, 1844(1 Pt A), 63–76.spa
dc.relation.referencesPingitore, V. E., Salvucci, E., Sesma, F., & Nader-Macías, M. E. (2007). Different strategies for purification of antimicrobial peptides from Lactic Acid Bacteria ( LAB ). Communicating Current Research and Educational Topics and Trends in Applied Microbiology, 21, 557– 568.spa
dc.relation.referencesQingsheng, L. S., & Jiaquan, Z. (1993). Study of Biogas Fermentation Liquid on Control of Bacterial Blight of Rice and Sheath and Culm Blight of Rice. China Biogas, 11(3), 11–15.spa
dc.relation.referencesRas, M., Girbal-Neuhauser, E., Paul, E., Spérandio, M., & Lefebvre, D. (2008). Protein extraction from activated sludge: An analytical approach. Water Research, 42(8-9), 1867– 1878.spa
dc.relation.referencesReddy, K. V. R., Yedery, R. D., & Aranha, C. (2004). Antimicrobial peptides: Premises and promises. International Journal of Antimicrobial Agents, 24, 536–547.spa
dc.relation.referencesRocha, G. F., Kise, F., Rosso, A. M., & Parisi, M. G. (2013). Péptidos con actividad antimicrobiana obtenidos de proteínas lácteas con extractos de salpichroa origanifolia. Informacion Tecnologica, 24(2), 23–30.spa
dc.relation.referencesSang, Y., & Blecha, F. (2012). Antimicrobial peptides and bacteriocins : alternatives to traditional Antimicrobial peptides and bacteriocins : alternatives to traditional antibiotics, (November 2008).spa
dc.relation.referencesSaraphirom, P., & Reungsang, A. (2011). Biological hydrogen production from sweet sorghum syrup by mixed cultures using an anaerobic sequencing batch reactor (ASBR). International Journal of Hydrogen Energy, 36(14), 8765–8773.spa
dc.relation.referencesSatoh, H., Oshima, K., Suda, W., Ranasinghe, P., Li, N., Gunawardana, E. G. W., … Mino, T. (2012). Bacterial Population Dynamics in a Laboratory Activated Sludge Reactor Monitored by Pyrosequencing of 16S rRNA. Microbes and Environments, 28(1), 65–70.spa
dc.relation.referencesSchägger, H., & von Jagow, G. (1987). Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Analytical biochemistry, 166(2), 368–379.spa
dc.relation.referencesSchink, B. (1997). Energetics of syntrophic cooperation in methanogenic degradation. Microbiology and molecular biology reviews : MMBR, 61(2), 262–280.spa
dc.relation.referencesSchrader, M., & Schulz-Knappe, P. (2001). Peptidomics technologies for human body fluids. Trends in Biotechnology, 19(10 Suppl), S55–60.spa
dc.relation.referencesScrivener, E., Barry, R., Platt, A., Calvert, R., Masih, G., Hextall, P., … Terrett, J. (2003). Peptidomics: A new approach to affinity protein microarrays. Proteomics, 3(2), 122–8.spa
dc.relation.referencesSharma, V. K., & Hobson, P. N. (1986). Interactions among cellulolytic bacteria from an anaerobic digester. Microbial ecology, 12(4), 343–53.spa
dc.relation.referencesShaw, C., & Verhaert, P. D. E. M. (2007). Peptidomics. (M. Soloviev, C. Shaw, & P. Andrn, Eds.), Peptidomics: Methods and Applications. Hoboken, NJ, USA: John Wiley & Sons, Inc.spa
dc.relation.referencesShen, D. (1997). Microbial diversity and application of microbial products for agricultural purposes in China. Agriculture, Ecosystems & Environment, 62(2-3), 237–245.spa
dc.relation.referencesShrout, J. D., & Nerenberg, R. (2012). Monitoring bacterial twitter: does quorum sensing determine the behavior of water and wastewater treatment biofilms? Environmental science & technology, 46(4), 1995–2005.spa
dc.relation.referencesSoloviev, M. (2010). Peptidomics: divide et impera. Methods in molecular biology (Clifton, N.J.), 615, 3–9.spa
dc.relation.referencesTanaka, S., Fujita, Y., Parry, H. E., Yoshizawa, A. C., Morimoto, K., Murase, M., … Tanaka, K. (2014). Mass++: A Visualization and Analysis Tool for Mass Spectrometry. Journal of proteome research, 13(8), 3846–3853.spa
dc.relation.referencesTao, X., Shang, B., Dong, H., Chen, Y., & Xin, H. (2014). Effects of Digestate from Swine Manure Digester on in Vitro Growth of Crop Fungal Pathogens: A Laboratory Study. Transactions of the ASABE, 1803–1810.spa
dc.relation.referencesTurcotte, C., Lacroix, C., Kheadr, E., Grignon, L., & Fliss, I. (2004). A rapid turbidometric microplate bioassay for accurate quantification of lactic acid bacteria bacteriocins. International Journal of Food Microbiology, 90(3), 283–293.spa
dc.relation.referencesWeiland, P. (2010). Biogas production: Current state and perspectives. Applied Microbiology and Biotechnology, 85, 849–860.spa
dc.relation.referencesWenig, P., & Odermatt, J. (2010). OpenChrom: a cross-platform open source software for the mass spectrometric analysis of chromatographic data. BMC bioinformatics, 11, 405.spa
dc.relation.referencesWilmes, P., & Bond, P. L. (2004). The application of two-dimensional polyacrylamide gel electrophoresis and downstream analyses to a mixed community of prokaryotic microorganisms. Environmental Microbiology, 6(9), 911–920.spa
dc.relation.referencesWilmes, P., Wexler, M., & Bond, P. L. (2008). Metaproteomics provides functional insight into activated sludge wastewater treatment. PloS one, 3(3), e1778.spa
dc.relation.referencesYang, D., Fan, X., Shi, X., Lian, S., Qiao, J., & Guo, R. (2014). Metabolomics reveals stagespecific metabolic pathways of microbial communities in two-stage anaerobic fermentation of corn-stalk. Biotechnology letters, 36(7), 1461–8.spa
dc.relation.referencesYu, F. B., Li, X. D., Ali, S. W., Song, C. F., Shan, S. D., & Luo, L. P. (2014). Use of biogas slurry for enhancing control of phytopathogens. Polish Journal of Environmental Studies, 23(2), 533–540.spa
dc.relation.referencesZiemiński, K., & Frąc, M. (2012). Methane fermentation process as anaerobic digestion of biomass: Transformations, stages and microorganisms. African Journal of Biotechnology, 11(18), 4127–4139.spa
dc.rightsDerechos Reservados - Universidad de los Llanos, 2016spa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.coarhttp://purl.org/coar/access_right/c_abf2spa
dc.rights.licenseAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)spa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/spa
dc.subject.armarcCompuestos orgánicos
dc.subject.armarcÁcidos orgánicos
dc.subject.proposalDigestión anaerobiaspa
dc.subject.proposalPéptidosspa
dc.subject.proposalActividad antimicrobianaspa
dc.subject.proposalMétodos de extracciónspa
dc.subject.proposalAnaerobic digestioneng
dc.subject.proposalPeptideseng
dc.subject.proposalExtraction methodseng
dc.subject.proposalAntimicrobial activityeng
dc.titleObtención de péptidos con posible bioactividad a partir de lodos digeridos derivados de la biodigestión anaerobia de aguas residualesspa
dc.typeTrabajo de grado - Pregradospa
dc.type.coarhttp://purl.org/coar/resource_type/c_7a1fspa
dc.type.coarversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/bachelorThesisspa
dc.type.redcolhttps://purl.org/redcol/resource_type/TPspa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
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Tesis y Trabajos de Grado