Persona: Velasco Santamaría, Yohana María
Proyectos de investigación
Unidades organizativas
Puesto de trabajo
Profesora Titular - Directora Técnica de Investigaciones
Apellidos
Velasco Santamaría
Nombre de pila
Yohana María
Dirección de correo electrónico
ORCID
0000-0002-6845-1663
Google Scholar
Fecha de nacimiento
1977-09-25
Grupo de investigación
2 resultados
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Publicación Acceso abierto Ecotoxicology of polycyclic aromatic hydrocarbons and petroleum wastewater in the orinoquia region(Society of Environmental Toxicology and Chemistry (SETAC) Latin America 15 Biennial Meeting, 2023-09-20) Calderón-Delgado, Ivonne; Velasco Santamaría, Yohana María; Corredor-Santamaría, WilsonPolycyclic aromatic hydrocarbons (PAH) are ubiquitous and characterized by low solubility, persistence in the environment, bioaccumulation in biota and high toxicity. Aquatic systems receive PAH through atmospheric deposition and direct runoff release, with the organisms that inhabit them being their main targets. Among these, fish are of particular interest because they occupy key positions in aquatic and terrestrial food webs. Petroleum extraction is one of the most relevant sectors in Colombia's economy; however, the environmental impact due to oil extraction and spillage accidents has generated contamination of soils and water sources, mortality of fauna and flora or changes in the dynamics of natural ecosystems, among others. Biomonitoring of oil pollution using aquatic organisms, especially native species has a great ecological relevance. In this sense, fish are considered as one of the main sentinel species to assess the state of aquatic ecosystems because they are ubiquitous in most aquatic environments exposed to contaminants and also because of their ecological relevance. Our data show alterations in fish from oil industry wastewater discharge sites and fish exposed to different PAH. In fact, concentrations of 250 and 70 ng/L of naphthalene (NAP) and phenanthrene (PHE), respectively, were recorded in rivers exposed to the discharge of produced water. Subacute exposures to sublethal concentrations of NAP 100 µg/g, PHE 50 µg/g and benzo[a]pyrene (BaP) 10 µg/g were evaluated. The results shown presence of genotoxicity in peripheral blood erythrocytes and nuclear abnormalities being higher in fish exposed to PHE, followed by BaP, and NAP, alteration in antioxidant response, fish exposed to BaP showed a significant increase in 7ethoxyresorufin-O-deethylase activity compared to the control, and presence of deleterious changes in tissue architecture such as hyperemia, degeneration of the nuclei, cytoplasmic degeneration, and vacuolization that could lead in the long term to a decline in fish populations exposed to these conditions. In conclusion, the results show that native Colombian fish such as Aequidens metae, Astyanax gr. bimaculatus and Piaractus orinoquencis are good sentinels of freshwaters potentially contaminated by hydrocarbons or in laboratory studies aimed at understanding the impact of petroleum compounds. Acknowledgment: This study was funded by Colciencias-ANH and Universidad de los Llanos Project number 112272151869, grant 721 – 2015.Publicación Acceso abierto Hepatic transcriptomic response in aequidens metae to Polycyclic Aromatic Hydrocarbons (PAH)(Society of Environmental Toxicology and Chemistry (SETAC) Latin America 14 Biennial Meeting, 2021-09-29) Corredor-Santamaría, Wilson; Mulet, Maite de María; Pinzón-Velasco, Andrés M.; Arbel, Ziv; Velasco Santamaría, Yohana MaríaAquatic systems receive Polycyclic aromatic hydrocarbons (PAH) through atmospheric deposition and direct runoff release, being fish one of the targets with a particular interest because they occupy key positions in aquatic and terrestrial food webs. In fact, exposure to different PAH can generate specific responses in gene expression profiles associated with biotransformation that could be used as biomarkers of exposure to these hydrocarbons. In this regard, RNA sequencing (RNA-seq) has provided insight into response mechanisms in gene expression studies by integrating data on biological effects in response to sublethal PAH exposure in non-model organisms without available genomic information. However, there are no transcriptomic profiling studies in fish in Colombia. Therefore, the objective of the present study was to characterize the hepatic transcriptional response in the non-model fish Aequidens metae exposed to polycyclic aromatic hydrocarbons under acute exposure.Liver samples were taken from adult specimens exposed via intraperitoneal to six conditions with five replicates, no injection control, solvent vehicle control, ßnaphthoflavone (BNF), naphthalene (NAP), phenanthrene (PHE) and benzo[a]pyrene (BaP), transcriptomic analysis of RNA-seq derived data was performed. A total of 396 differentially expressed genes (DEG) were identified for the comparison between solvent and BNF, 330 for NAP, 396 for PHE and 381 for BaP of which 15 genes were common to all 4 PAH. In turn, enrichment analysis identified 9, 31, 12 and 31 KEGG pathways for BNF, NAP, PHE and BaP, respectively. In general, the pathways affected were associated with the processes of cell growth and death; lipid and carbohydrate metabolism; transport and catabolism; folding, sorting and degradation; the endocrine system; signal transduction; xenobiotic metabolism; nucleotide metabolism; viral infectious diseases and the endocrine system. Overall, the analysis showed evidence of toxic effects on liver tissue in fish exposed to the different PAH underlying molecular mechanisms through the study of transcriptomic profiling being a pioneer study in Colombian fish in this area. Acknowledgment: This study was funded by Colciencias-ANH and Universidad de los Llanos project No 162-2016 and also a Project from the Pontificia Universidad Javeriana from Colombia.