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Análisis de expresión genética en la respuesta de defensa de la yuca (Manihot Esculenta Crantz) mediada por el ácido salicílico contra el ataque del ácaro verde (Mononychellus Tanajoa) y mosca blanca (Aleurotrachelus Socialis)

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dc.contributor.advisorMarín Colorado, Jaime Albertospa
dc.contributor.authorValencia Londoño, Alejandrospa
dc.date.accessioned2019-11-05T19:37:50Zspa
dc.date.available2019-11-05T19:37:50Zspa
dc.descriptionIncluye tablas y figuras spa
dc.description.abstractLa yuca (Manihot esculenta) es uno de los cultivos más importantes para la alimentación de la región tropical debido a su rico contenido de hidratos de carbono. Sin embargo, ésta se ve afectada por diferentes factores bióticos y abióticos como el ácaro verde (Mononychellus tanajoa) y la mosca blanca (Aleurotrachellus socialis), los cuales generan pérdidas significantes en la producción de la yuca. Es por esto que las plantas han desarrollado muchas estrategias diferentes como mecanismo de defensa para hacer frente a estos tipos de estrés como la expresión de genes los cuales generan diferentes respuestas de defensa en la planta. El análisis de expresión de genes es una herramienta que permite conocer cómo se comportan los genes frente al estrés, para esto se realizó una búsqueda de genes de referencia y genes relacionados con el ácido salicílico con el programa Genevestigator (RefGenes, Pertubations) para elegir cuales presentan mejor regulación frente al ataque, luego de esto se diseñaron los primers con la herramienta Primer 3 para amplificar las regiones deseadas.spa
dc.description.abstractCassava (Manihot esculenta) is one of the most important crops for food in the tropical region due to its rich carbohydrate content. However, it is affected by different biotic and abiotic factors such as the green mite (Mononychellus tanajoa) and the whitefly (Aleurotrachellus socialis), which generate significant losses in cassava production. This is why plants have developed many different strategies as a defense mechanism to deal with these types of stress, such as the expression of genes which generate different defense responses in the plant. Gene expression analysis is a tool that allows us to know how genes behave in the face of stress. For this, a search for reference genes and genes related to salicylic acid was carried out with the Genevestigator program (RefGenes, Pertubations) to choose which ones. They present better regulation against the attack, after this the primers were designed with the Primer 3 tool to amplify the desired regions.eng
dc.description.degreelevelPregradospa
dc.description.degreenameBiologíaspa
dc.description.notesTrabajo de grado presentado como requisito parcial para optar al título de Biólogo spa
dc.description.tableofcontents 1. Introducción -- 2. Planteamiento Del Problema -- 3. Hipótesis -- 4. Objetivos -- 4.1 Objetivo general -- 4.2 Objetivos específicos -- 5. Justificación -- 6. Marco teórico -- 6.1 Los cultivos y su defensa contra las plagas -- 6.2 Ácido salicílico -- 6.3 Artrópodos plaga -- 6.3.1 Aleurotrachelus socialis -- 6.3.2 Mononychellus tanajoa -- 6.4 Oviposición -- 6.5 Regiones promotoras de los genes --7. Metodología y área de estudio -- 7.1 Material biológico -- 7.2 Colecta de artrópodos plaga -- 7.3 Ensayos a libre y no libre escogencia -- 7.3.1 Bioensayo ácaro verde (M. tanajoa) -- 7.3.2 Bioensayo mosca blanca (A. sociales) -- 7.4 Análisis molecular de la interacción planta-artrópodo --7.5 Extracción de ADN Y ARN -- 7.6 Cuantificación y gel de calidad -- 7.7 Síntesis de cDNA -- 7.8 Búsqueda de Genes de referencia y genes relacionados con la vía del ácido salicílico -- 7.9 PCR y diseño de primers -- 7.10 Caracterización estructural de los promotores -- 7.11 Análisis estadístico -- 8. Resultados y discusión -- 8.1 Oviposición Mononychellus tanajoa -- 8.1.1 No libre escogencia 8.1.2 Libre escogencia -- 8.2 Caracterización estructural de los promotores -- 8.3 Genes de referencia y genes relacionados con la vía del ácido salicílico -- 8.4 PCR primers G3PDH y 18s -- 9. Conclusiones -- 10. Bibliografíaspa
dc.format.extent64 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.citationValencia Londoño, Alejandro (2018). Análisis de expresión genética en la respuesta de defensa de la yuca (Manihot Esculenta Crantz) mediada por el ácido salicílico contra el ataque del ácaro verde (Mononychellus Tanajoa) y mosca blanca (Aleurotrachelus Socialis) [Trabajo de grado, Universidad de los Llanos]. Repositorio digital Universidad de los Llanos.spa
dc.identifier.instnameUniversidad de los Llanosspa
dc.identifier.reponameRepositorio digital Universidad de los Llanosspa
dc.identifier.repourlhttps://repositorio.unillanos.edu.cospa
dc.identifier.urihttps://repositorio.unillanos.edu.co/handle/001/1471spa
dc.language.isospaspa
dc.publisherUniversidad de los Llanosspa
dc.publisher.branchSede Barcelonaspa
dc.publisher.facultyFacultad de ciencias básicas e ingenieríaspa
dc.publisher.placeVillavicenciospa
dc.publisher.programBiologíaspa
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dc.subject.proposalÁcido salicílicospa
dc.subject.proposalArtrópodos plagasspa
dc.subject.proposalManihot esculenta
dc.subject.proposalPromotores
dc.subject.proposalOviposición
dc.titleAnálisis de expresión genética en la respuesta de defensa de la yuca (Manihot Esculenta Crantz) mediada por el ácido salicílico contra el ataque del ácaro verde (Mononychellus Tanajoa) y mosca blanca (Aleurotrachelus Socialis) spa
dc.typeTrabajo de grado - Pregradospa
dc.type.categoryProyectos de investigaciónspa
dc.type.coarhttp://purl.org/coar/resource_type/c_7a1fspa
dc.type.coarversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/bachelorThesisspa
dc.type.redcolhttps://purl.org/redcol/resource_type/TPspa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dspace.entity.typePublication

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