Icono govco
 

Publicación:
Efecto de los errores en la inseminación con semen congelado sobre la morfofisiología espermática bovina

Vista sencilla de ítem
dc.contributor.authorVilla-Duque, Norbertospa
dc.contributor.authorValencia-Giraldo, Julián A.spa
dc.contributor.authorGómez-Londoño, Germánspa
dc.contributor.authorHenao-Uribe, Francisco J.spa
dc.date.accessioned2016-07-01T00:00:00Z
dc.date.accessioned2024-07-25T18:14:12Z
dc.date.available2016-07-01T00:00:00Z
dc.date.available2024-07-25T18:14:12Z
dc.date.issued2016-07-01
dc.description.abstractEn el presente trabajo se evaluó la criopreservación a -196°C, la descongelación y la aplicación del semen, en 32 ganaderías bovinas del centro de Colombia, y se estudió, in vitro, el efecto de los errores encontrados sobre la integridad de las membranas y la morfología espermáticas. La recolección de información en fincas se realizó mediante formulario específico. El estudio in vitro se ejecutó en el Instituto de Biotecnología Agropecuaria de la Universidad de Caldas (Manizales, Colombia), y consistió en someter pajillas comerciales de 0.5 ml (30x106 epzs), de toros Holstein, Jersey y Pardo Suizo, a la técnica instrumental convencional y a cinco modificaciones de la misma (errores más frecuentes), mediante un arreglo factorial 6x3 en diseño completamente al azar. En ninguna de las fincas evaluadas se aplicó correctamente la práctica de la inseminación artificial (IA). Los errores más frecuentes fueron: deterioro de la estructura externa y condiciones inadecuadas de almacenamiento de los tanques, almacenamiento de pajillas en la canastilla, y manipulación de pajillas por fuera del cuello del tanque, descongelación en la axila, en agua refrigerada y en agua no potable. La integridad acrosómica (IAs) y la integridad estructural de la membrana espermática (IEM) fueron afectadas (P<0.05) por la interacción genotipo x técnica instrumental. La resistencia de las membranas espermáticas fue afectada (P<0.01) por el genotipo y la técnica instrumental. Los valores más bajos para integridad y resistencia de membranas correspondieron al semen de Pardo Suizo descongelado en la axila y almacenado con bajo nivel de nitrógeno. A pesar de que los porcentajes de gotas citoplásmicas y colas en látigo (<7% y <11.3%, respectivamente) fueron superiores a lo esperado, el porcentaje de espermatozoides normales estuvo siempre por encima del 70%, indicativo de que ninguno de los factores evaluados afectó de manera determinante la morfología espermática.Palabras clave: análisis de semen; espermatozoide bovino; inseminación artificial; preservación de semen; semen analysis; bovine spermatozoaspa
dc.description.abstractThis work evaluated cryopreservation at -196°C, thawing and semen insemination in 32 bovine herds from the center of Colombia; it also studied, in vitro, the effect of the errors found regarding membrane integrity and sperms morphology. A specific form was used for collecting information from farms. The in vitro study was carried out at the Universidad de Caldas’ Agricultural Biotechnology Institute (Instituto de Biotecnología Agropecuaria, Manizales, Colombia); it consisted of using commercial 0.5 ml straws (30x106 epzs) containing sperm from Holstein, Jersey and Brown Swiss bulls, comparing conventional handling technique to five modifications of it (most frequent errors) using a completely randomised 6x3 factorial design. Artificial insemination (AI) was not used correctly on any of the farms evaluated here. The most frequently occurring errors were deterioration of external structure and inadequate storage conditions for the tanks, storing straws in the bucket and manipulating straws outside the tank neck, thawing in the armpit, in refrigerated water and in non-potable water. Acrosome integrity  artificial insemination, semen preservation. (AsI) and sperm membrane integrity (SMI) were affected (p<0.05) by genotype x handling technique interaction. Sperm membrane resistance was affected (p<0.01) by genotype and handling technique. Brown Swiss semen thawed in the armpit and stored with low nitrogen level had the lowest values for membrane integrity and resistance. In spite of cytoplasmic droplet and coiled tail percentages being greater than expected (<7% and <11.3%, respectively), normal sperm percentage was always above 70%, indicating that none of the factors evaluated here decisively affected sperm morphology.Key words: semen analysis; bovine spermatozoaeng
dc.format.mimetypetext/htmlspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.doi10.22579/20112629.326
dc.identifier.eissn2011-2629
dc.identifier.issn0121-3709
dc.identifier.urihttps://repositorio.unillanos.edu.co/handle/001/3831
dc.identifier.urlhttps://doi.org/10.22579/20112629.326
dc.language.isospaspa
dc.publisherUniversidad de los Llanosspa
dc.relation.bitstreamhttps://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/326/900
dc.relation.bitstreamhttps://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/326/pdf_17
dc.relation.citationendpage55
dc.relation.citationissue1spa
dc.relation.citationstartpage46
dc.relation.citationvolume20spa
dc.relation.ispartofjournalOrinoquiaspa
dc.relation.referencesAl-Makhzoomi A, Lundeheim N, Haard M, Rodriguez-Martinez H. Sperm morphology and fertility of progeny-tested AI dairy bulls in Sweden. Theriogenology. 2008;70(4):682-691.spa
dc.relation.referencesAlmquist JO, Grube KE, Rosenberger JL. Effect of thawing time on fertility of bovine spermatozoa in French straws. J Dairy Sci.1982;65(5):824-827.spa
dc.relation.referencesAnzar M, Kroetsch T, Boswall L. Cryopreservation of bull semen shipped overnight and its effect on post-thaw sperm motility, plasma membrane integrity, mitochondrial membrane potential and normal acrosomes. Anim Reprod Sci.2011;126(1-2):23-31.spa
dc.relation.referencesÁvila-Portillo LM, Madero JI, López C, León MF, Acosta L, et al. Fundamentos de criopreservación. Rev Colomb Obstet Ginecol. 2006;57 (4):291-300.spa
dc.relation.referencesBarth AD, Waldner CL. Factors affecting breeding soundness classification of beef bulls examined at the Western College of Veterinary Medicine. The Canadian veterinary journal La revue veterinaire canadienne.2002;43(4):274-284.spa
dc.relation.referencesBerndtson WE, Pickett BW. 1978. Thecniques for the cryopreservation and fiel handling of bovine spermatozoa. NAO Sciences editor.spa
dc.relation.referencesBrackett BG, Oliphant G. Capacitation of rabbit spermatozoa in vitro. Biol Reprod.1975;12(2):260-274.spa
dc.relation.referencesBrown JL, Senger PL, Hillers JK. Influence of thawing time and postthaw temperature on acrosomal maintenance and motility of bovine spermatozoa frozen in .5-ml French straws. J Anim Sci. 1982;54(5):938-944.spa
dc.relation.referencesCampbell RC, Hancock JL, Rothschild L. Counting live and dead bull spermatozoa. J Exp Biol. 1953;30:44-49.spa
dc.relation.referencesCarreira JT, Mingoti GZ, Rodrigues LH, Silva C, Perri SH, Koivisto MB. Impact of proximal cytoplasmic droplets on quality traits and in-vitro embryo production efficiency of cryopreserved bull spermatozoa. Acta Vet Scand.2012;54:1.spa
dc.relation.referencesCastillo R, Morales AM, Carrasco A. Guia de uso de la criocirugia en atencion primaria. Medicina de Familia (And). 2002;3(2);114122.spa
dc.relation.referencesCorrea JR, Rodriguez MC, Patterson DJ, Zavos PM. Thawing and processing of cryopreserved bovine spermatozoa at various temperatures and their effects on sperm viability, osmotic shock and sperm membrane functional integrity. Theriogenology. 1996;46(3):413-420.spa
dc.relation.referencesDiaz O, Mesa H, Gómez G, Henao FJ. Evaluación invitro de la viabilidad del semen porcino hasta 120 horas de almacenamiento en refrigeración. Veterinaria y Zootecnia. 2009;3:32-37.spa
dc.relation.referencesFoote RH. The history of artificial insemination: Selected notes and notables. J Anim Sci. 2002;80:1-10.spa
dc.relation.referencesHallap T, Haard M, Jaakma U, Larsson B, Rodriguez-Martinez H. Does cleansing of frozen-thawed bull semen before assessment provide samples that relate better to potential fertility? Theriogenolog. 2004;62(3-4):702-713.spa
dc.relation.referencesKasimanickam R, Nebel RL, Peeler ID, Silvia WL, Wolf KT, et al. Breed differences in competitive indices of Holstein and Jersey bulls and their association with sperm DNA fragmentation index and plasma membrane integrity. Theriogenology. 2006;66(5):1307-1315.spa
dc.relation.referencesMazur P. Freezing of living cells: mechanisms and implications. Am J Physiol. 1984;247(3 Pt 1):C125-142.spa
dc.relation.referencesMendoza JA, Dulin P, Warren T. The lower hydrolysis of ATP by the stress protein GroEL is a major factor responsible for the diminished chaperonin activity at low temperature. Cryobiolog.2000;41(4):319-323.spa
dc.relation.referencesNebel RL. 1997. Thechniques for Artificial Insemination of cattle whith frozen-thawed semen. RS Youngquist editor. En: Current therapy in large animal theriogenology p. 251-256.spa
dc.relation.referencesNur Z, Kamuran II, Ak K. Effects of different temperature treatments applied to deep stored bull semen on post-thaw cold shocked spermatozoa. Bull Vet Inst Pulawy. 2006;50:79-83.spa
dc.relation.referencesO’Flaherty C, Beorlegui N, Beconi MT. Participation of superoxide anion in the capacitation of cryopreserved bovine sperm. Int J Androl.2003;26(2):109-114.spa
dc.relation.referencesOsorio-Serna RE, Giraldo JF, Mesa H, Gómez G, Henao FJ. Evaluación de la integridad acrosómica en semen de verraco. Veterinaria y Zootecnia. 2007;1:41-47.spa
dc.relation.referencesPace MM, Sullivan JJ, Elliott FI, Graham EF, Coulter GH. Effects of thawing temperature, number of spermatozoa and spermatozoal quality on fertility of bovine spermatozoa packaged in 5 ml french straws. J Anim Sci.1981;53:693-701.spa
dc.relation.referencesPerez-Llano B, Yenes-Garcia P, Garcia-Casado P. Four subpopulations of boar spermatozoa defined according to their response to the short hypoosmotic swelling test and acrosome status during incubation at 37 degrees C. Theriogenology.2003;60(8):1401-1407.spa
dc.relation.referencesPickett BW, Berndtson WE, Sullivan JJ. Influence of seminal additives and packaging systems on fertility of frozen bovine spermatozoa. J Anim Sci.1978;47(Suppl 2):12-47.spa
dc.relation.referencesPursel VG, Johnson LA. Glutaraldehyde fixation of boar spermatozoa for acrosome evaluation. Theriogenology. 1974; 1(2):63-68.spa
dc.relation.referencesRisopatron J, Sanchez R, Sepulveda N, Villagran E, Peña P. 1994. Seleccion de espermatozoides de bovino desde semen congelado-descongelado.Comparacion de dos metodos. Archivos de Medicina Veterinaria (Valdivia). 1994;26(1):25-40.spa
dc.relation.referencesRodriguez OL, Berndtson WE, Ennen BD, Pickett BW. Effect of rates of freezing, thawing and level of glycerol on the survival of bovine spermatozoa in straws. J Anim Sci. 1975;41(1):129-136.spa
dc.relation.referencesRubio-Guillen J, González D, González Y, Madrid-Bury N, QuinteroMoreno A. Puede el ORT complementar las pruebas clasicas de valoracion seminal y predecir la fertilidad en toros. Revista Luz. 2007;1-5.spa
dc.relation.referencesSaacke R. Fertilidad del toro: una opinion sobre su estado actual y perspectivas. Taurus, BsAs.2003;5:18-28.spa
dc.relation.referencesSpitzer J. 2000. Bull Breeding Soundness Evaluation: Current Status. IN Chenoweth P.J. International Veterinary Information Service editor. En: Topics in Bull Fertility. Manhattan, Kansas, USA.spa
dc.relation.referencesTroxel TR. 2007. Purebred cattle series: artificial insemination. University of Arkansas Division of Agriculture, Cooperative Extension Service, FSA3118.spa
dc.relation.referencesVeznik Z, Svecova D, Zajicova A, Reckova Z, J. R. The interrelationship between quality parameters of sperm before and after separation by gradient centrifugation. Veterinarni Medicina.2007;52(10):423–429.spa
dc.relation.referencesWatson PF. The causes of reduced fertility with cryopreserved semen. Animal reproduction science. 2000;60-61:481-492.spa
dc.relation.referencesWoods EJ, Benson JD, Agca Y, Critser JK. Fundamental cryobiology of reproductive cells and tissues. Cryobiology.2004;48(2):146-156.spa
dc.rightsOrinoquia - 2016spa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.coarhttp://purl.org/coar/access_right/c_abf2spa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/spa
dc.sourcehttps://orinoquia.unillanos.edu.co/index.php/orinoquia/article/view/326spa
dc.subjectanálisis de semenspa
dc.subjectespermatozoide bovinospa
dc.subjectinseminación artificialspa
dc.subjectpreservación de semenspa
dc.subjectsemen analysisspa
dc.subjectbovine spermatozoaspa
dc.subjectsemen analysiseng
dc.subjectbovine spermatozoaeng
dc.titleEfecto de los errores en la inseminación con semen congelado sobre la morfofisiología espermática bovinaspa
dc.title.translatedThe effect of errors regarding frozen semen insemination on bovine sperm morphophysiologyeng
dc.typeArtículo de revistaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.type.coarversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.localJournal articleeng
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dspace.entity.typePublicationspa

Archivos

Colecciones

Revista Orinoquia