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Variedad bacteriana en cultivos piscícolas y su resistencia a antibacterianos

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dc.contributor.authorParrado, Maritzaspa
dc.contributor.authorSalas, María Clemenciaspa
dc.contributor.authorHernández-Arévalo, Gilmaspa
dc.contributor.authorOrtega, Patriciaspa
dc.contributor.authorYossa, Martha I.spa
dc.date.accessioned2014-09-01 00:00:00
dc.date.accessioned2022-06-13T17:41:59Z
dc.date.available2014-09-01 00:00:00
dc.date.available2022-06-13T17:41:59Z
dc.date.issued2014-09-01
dc.description.abstractPara determinar la variedad y resistencia frente a antibacterianos, durante un periodo productivo en el cual no se utilizaron antibióticos, en dos piscícolas del departamento del Meta, se obtuvieron muestras de agua y sedimento de donde se aislaron bacterias pertenecientes al grupo de heterótrofas Gram negativas. Las muestras se sembraron en medios selectivos Agar TCBS, McConkey, Citófaga, Cetrimide y Plate Count Agar para hacer recuentos bacterianos con las Técnicas de Análisis Microbiológico de la ICMSF y el Standart Methods. Las colonias diferentes de cada medio se repicaron para identificación bioquímica con kit comercial BBL-cristal®, y para el estudio de resistencia a seis antibacterianos se empleó el procedimiento descrito por Kirby-Bauer. La multi-resistencia, se estimó con el índice resistencia múltiple a antibióticos (MAR), y el índice de abundancia relativa (IAR) se calculó de acuerdo con Latorre (2007). Se identificaron 19 cepas bacterianas, seis correspondieron a Klebsiella pneumoniae, cinco a Enterobacter sp y cloacae, y las demás a Pseudomonas fluorescens y aerouginosa, Chryseobacterium sp. y gleum, Sphingobacterium multivorum, Serratia marcescens y Tetrathiobacter mimigardefordensis. Todas las cepas aisladas fueron resistentes a Eritromicina y el mayor índice de multi- resistencia lo presentó Enterobacter cloacae con 1.00 y 0.8. El mayor IAR correspondió a enterobacterias (≥800ufc/g). El encontrar seis cepas de Klebsiella pneumonidae y cinco de Enterobacter spcondiferentes índices de resistencia en los dos ambientes analizados, indica la gran variabilidad de estas especies. La resistencia de todos los microorganismos a Eritromicina, y el alto IMR de Enterobacter, demuestran que el uso de antibióticos en acuicultura, sumado a la alta concentración de enterobacterias puede tener efectos ambientales adversos que podrían repercutir en la salud pública.spa
dc.description.abstractBacterial aerobic gram negative heterotrophic community was isolated from water and sediment to determine the variety and antibacterial resistance during a productive period in which no antibiotics were used in two fish farms of Meta country. Samples were plated on selective medium TCBS Agar, McConkey, Citófaga, Cetrimide and Plate Count Agar toperform bacterial counts with the Technical Analysis of Microbiological ICMSF and the Standard Methods. The different colonies were replicated to each medium biochemical identification BBL-Cristal® commercial kit, and for the study of the six antibacterial resistance was used the procedure described by Kirby -Bauer. The multi - resistance was estimated by the multiple antibiotic resistance index (MAR) and the relative abundance index (RAI) was calculated according to Latorre (2007).19 bacterial strains were identified, six were for Klebsiella pneumoniaeand five Enterobacter sp cloacae, and others: aerouginosa Pseudomonas and fluorescens, Chryseobacterium sp. and gleum, Sphingobacterium multivorum, Serratia marcescens and Tetrathiobacter mimigardefordensis. All isolates were resistant to erythromycin and the highest rate of multi- resistant Enterobacter cloacae presented him with 1.00 and 0.8. The biggestRAI accounted enterobacteria (≥800 cfu/g). Finding six strains of Klebsiella pneumonidae five Enterobacter sp with different resistance rates in both environments analyzed, indicates the great variability of these species. The resistance of all microorganisms to erythromycin, and high MAR Enterobacter show that the use of antibiotics in aquaculture coupled with the high concentration of enterobacter may have adverse environmental impacts that could affect public health.eng
dc.format.mimetypeapplication/pdfspa
dc.identifier.doi10.22579/20112629.382
dc.identifier.eissn2011-2629
dc.identifier.issn0121-3709
dc.identifier.urihttps://repositorio.unillanos.edu.co/handle/001/2617
dc.identifier.urlhttps://doi.org/10.22579/20112629.382
dc.language.isospaspa
dc.publisherUniversidad de los Llanosspa
dc.relation.bitstreamhttps://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/382/988
dc.relation.citationeditionNúm. 2 sup , Año 2014 : Vol. 18 Núm 2 sup (2014)spa
dc.relation.citationendpage246
dc.relation.citationissue2 supspa
dc.relation.citationstartpage238
dc.relation.citationvolume18spa
dc.relation.ispartofjournalOrinoquiaspa
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dc.rightsOrinoquia - 2014spa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.coarhttp://purl.org/coar/access_right/c_abf2spa
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/spa
dc.sourcehttps://orinoquia.unillanos.edu.co/index.php/orinoquia/article/view/382spa
dc.subjectBiodiversidadspa
dc.subjectservicio ecosistémicospa
dc.subjecturbanizaciónspa
dc.subjectcambio climáticospa
dc.subjectciencias básicasspa
dc.titleVariedad bacteriana en cultivos piscícolas y su resistencia a antibacterianosspa
dc.title.translatedBacterial variety of fish farms and resistance to antibacterialeng
dc.typeArtículo de revistaspa
dc.typeJournal Articleeng
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.type.coarversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.localSección Artículosspa
dc.type.localSección Articleseng
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dspace.entity.typePublication

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