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Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando

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dc.contributor.authorVejarano-Orjuela, Álvarospa
dc.contributor.authorHernández-Vásquez, Aurelianospa
dc.contributor.authorRondón-Barragán, Iang S.spa
dc.contributor.authorSuárez-Mesa, Rafael A.spa
dc.date.accessioned2020-05-11T00:00:00Z
dc.date.accessioned2024-07-25T18:15:00Z
dc.date.available2020-05-11T00:00:00Z
dc.date.available2024-07-25T18:15:00Z
dc.date.issued2020-05-11
dc.description.abstractEl gen del receptor para la hormona luteinizante (LHR-LHCGR) es un gen altamente conservado y su mRNA origina diferentes variantes por corte y empalme alternativo (splicing alternativo). En bovinos se han reportado variantes que traducen en proteína pero ésta no atraviesa la membrana celular; en humanos ante una deleción del exón 10 no hay respuesta a la acción de la LH, por lo tanto, es razonable asumir que las diferencias en la respuesta a la LH estén relacionadas con la expresión de los LHR. El objetivo de este trabajo fue comparar entre la raza criolla Colombiana Romosinuano y la raza Brahman los posibles polimorfismos del ARNm del gen como potenciales indicadores de fertilidad. Se tomaron 5 vacas ciclando de cada raza se extirpó el ovario que contenía un folículo preovulatorio, se retiró la pared folicular, se extrajo el RNA total y se sintetizó, amplificó, secuenció y comparó el ADNc contra secuencias ya reportadas, presentando una alta similitud. Se concluye que en estas razas la ausencia de variantes en la expresión del gen, estaría asociada a buen desempeño reproductivo.spa
dc.description.abstractThe luteinizing hormone (LH) choriogonadotropin receptor (LHCGR) gene is highly conserved and its mRNA causes variants by alternative splicing. Variants have been reported in cattle that translate into protein; however, this does not cross the cell membrane. Exon 10 deletion in humans does not trigger a response to the action of LH; it is therefore reasonable to assume that differences regarding response to LH are related to LHR expression. This work was aimed at comparing the Colombian creole Romosinuano breed to the Brahman breed regarding possible HCGR gene mRNA polymorphisms as potential fertility indicators. Five cycling cows were taken from each breed and the ovaries containing pre-ovulatory follicles were excised, the follicular wall was removed and total RNA extracted. The cDNA was synthesised, amplified, sequenced and compared to already reported sequences, great similarity being observed. It was concluded that the lack of variants regarding gene expression in these breeds would be associated with good reproductive performance.eng
dc.format.mimetypeapplication/pdfspa
dc.identifier.doi10.22579/20112629.590
dc.identifier.eissn2011-2629
dc.identifier.issn0121-3709
dc.identifier.urihttps://repositorio.unillanos.edu.co/handle/001/3974
dc.identifier.urlhttps://doi.org/10.22579/20112629.590
dc.language.isospaspa
dc.publisherUniversidad de los Llanosspa
dc.relation.bitstreamhttps://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/590/pdf
dc.relation.citationendpage51
dc.relation.citationissue1spa
dc.relation.citationstartpage42
dc.relation.citationvolume24spa
dc.relation.ispartofjournalOrinoquiaspa
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dc.rightsOrinoquia - 2020spa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.coarhttp://purl.org/coar/access_right/c_abf2spa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/spa
dc.sourcehttps://orinoquia.unillanos.edu.co/index.php/orinoquia/article/view/590spa
dc.subjectbovine creole reproductioneng
dc.subjectLHR–LHCGReng
dc.subjectfertility indicatoreng
dc.subjectbovinos criollo reproducciónspa
dc.subjectLHR–LHCGRspa
dc.subjectreprodução de bovinos crioulosspa
dc.subjectLHR–LHCGRspa
dc.titlePolimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclandospa
dc.title.translatedLuteinizing hormone choriogonadotropin receptor (LHGCR) gene polymorphism in ovarian follicles from cycling Romosinuano and Brahman cowseng
dc.typeArtículo de revistaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.type.coarversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.localJournal articleeng
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dspace.entity.typePublicationspa

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