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Seguridad biológica y ensayo in vitro de expresión génica en PBMC de caninos vacunados experimentalmente contra Toxocara canis

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dc.contributor.authorJaramillo-Hernández , Dumar Alexanderspa
dc.contributor.authorLesmes Rodriguez, Lida Carolinaspa
dc.contributor.authorAlcantara Neves, Neuza Mariaspa
dc.date.accessioned2023-08-27T00:00:00Z
dc.date.accessioned2024-09-23T20:46:45Z
dc.date.available2023-08-27T00:00:00Z
dc.date.available2024-09-23T20:46:45Z
dc.date.issued2023-08-27
dc.description.abstractLa toxocariasis es una zoonosis desatendida de alta prevalencia e impacto en la salud pública de poblaciones empobrecidas del mundo, su agente causal es el nematodo del género Toxacara. Actualmente se utilizan antiparasitarios de amplio espectro para afrontar la enfermedad, sin embargo, no existe una vacuna para prevenirla. El objetivo de esta investigación fue evaluar la seguridad biológica de ensayos clínicos experimentales de vacunología inversa; y así mismo evaluar la respuesta in vitro de PBMC de caninos previamente vacunados con las proteínas recombinantes rTcVcan y rTcCad de Toxocara canis, a través del ensayo de expresión génica medida por RT-qPCR de citocinas IL-5, IL- 6, IL-17A, IL-10, TNF-α e INF-Ɣ. Como resultado, una vez realizados los ensayos clínicos para la vacunación de los cachorros, no se observaron diferencias con respecto a los rangos de referencia de los análisis de hematología, así como de los análisis de las funciones hepática y renal en los cachorros y; por otra parte, en los ensayos de expresión génica en PBMC, únicamente el gen ACTB se expresó con éxito, los genes referentes a las citocinas no presentaron resultados estadísticamente significativos. En conclusión, las formulaciones aplicadas no fueron tóxicas y no tuvieron efectos adversos en los animales experimentales y los ensayos de expresión génica en PBMC de los cachorros vacunados con las proteínas recombinantes rTcVcan y rTcCad contra T. canis no presentaron resultados concluyentes con respecto al éxito de estos procedimientos en la primera fase de la vacuna experimental.spa
dc.description.abstractToxocariasis is a neglected zoonosis with a high prevalence and impact on the public health of impoverished populations in the world, its causative agent is the nematode of the genus Toxacara. Broad-spectrum antiparasitics are currently used to deal with the disease, however, there is no vaccine to prevent it. The objective of this research was to evaluate the biological safety of experimental clinical trials of reverse vaccinology; and also, to evaluate the in vitro response of PBMC of canines previously vaccinated with the recombinant proteins rTcVcan and rTcCad of Toxocara canis, through the gene expression assay measured by RT-qPCR of IL-5, IL-6, IL-17. , IL-10, TNF-α and INF-Ɣ cytokines. As result, once the clinical trials for the vaccination of the puppies were carried out, no differences were observed with respect to the reference ranges of the hematology analysis, as well as the analyzes of the liver and kidney functions in the puppies and; on the other hand, in the gene expression assays in PBMC, only the ACTB gene was successfully expressed, the cytokine genes did not present statistically significant results. In conclusion, the applied formulations were not toxic and had no adverse effects on the experimental animals and gene expression assays in PBMC of vaccinated puppies with the recombinant rTcVcan and rTcCad proteins against T. canis did not show conclusive results regarding the success of these procedures in the first phase of the experimental vaccine.eng
dc.format.mimetypeapplication/pdfspa
dc.identifier.doi10.22579/22484817.986
dc.identifier.eissn2248-4817
dc.identifier.urihttps://repositorio.unillanos.edu.co/handle/001/4382
dc.identifier.urlhttps://doi.org/10.22579/22484817.986
dc.language.isospaspa
dc.publisherUniversidad de los Llanosspa
dc.relation.bitstreamhttps://revistas.unillanos.edu.co/index.php/sistemasagroecologicos/article/download/986/1044
dc.relation.citationeditionNúm. 1 , Año 2023 : Enero-Juniospa
dc.relation.citationendpage21
dc.relation.citationissue1spa
dc.relation.citationstartpage2
dc.relation.citationvolume14spa
dc.relation.ispartofjournalRevista Sistemas de Producción Agroecológicosspa
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.coarhttp://purl.org/coar/access_right/c_abf2spa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/spa
dc.sourcehttps://revistas.unillanos.edu.co/index.php/sistemasagroecologicos/article/view/986spa
dc.subjectToxocariasiseng
dc.subjectReverse Vaccinologyeng
dc.subjectRecombinant Proteineng
dc.subjectBiosafetyeng
dc.subjectPBMCeng
dc.subjectToxocariasisspa
dc.subjectVacunología Inversaspa
dc.subjectProteína Recombinantespa
dc.subjectBioseguridadspa
dc.subjectPBMCspa
dc.subjectToxocaríasespa
dc.subjectVacinologia Reversaspa
dc.subjectProteína Recombinantespa
dc.subjectBiossegurançaspa
dc.subjectPBMCspa
dc.titleSeguridad biológica y ensayo in vitro de expresión génica en PBMC de caninos vacunados experimentalmente contra Toxocara canisspa
dc.title.translatedBiological safety and in vitro gene expression assay in PBMC of canines experimentally vaccinated against Toxocara caniseng
dc.typeArtículo de revistaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.type.coarversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.localJournal articleeng
dc.type.redcolhttp://purl.org/redcol/resource_type/ARTREFspa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dspace.entity.typePublicationspa

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