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Efeito do estresse pré-natal na regulação da inflamação alérgica pulmonar no modelo murino de asma experimental

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dc.contributor.authorGonçalves Jr, Vagnerspa
dc.contributor.authorLigeiro de Oliveira, Ana Paulaspa
dc.contributor.authorKieling, Karinspa
dc.date.accessioned2016-07-01 00:00:00
dc.date.accessioned2022-06-13T17:41:55Z
dc.date.available2016-07-01 00:00:00
dc.date.available2022-06-13T17:41:55Z
dc.date.issued2016-07-01
dc.description.abstractDevido ao seu rápido crescimento, o feto é particularmente vulnerável a insultos e modificações no millieu hormonal. Este fato sugere que situações adversas experimentadas pela mãe grávida podem alterar o desenvolvimento e a saúde da prole, explicado principalmente pela permeabilidade da barreira placentária a diversos hormônios e substâncias. O objetivo deste trabalho foi estudar o efeito do estresse pré-natal na regulação da inflamação alérgica pulmonar, empregando o modelo murino de asma experimental. Para este propósito foram utilizadas camundongas virgens da linhagem Swiss, com 50 dias de idade. Foi empregado o modelo de choque nas patas para promover o estresse pré-natal e o modelo do “metrô de Nova Efeito do estresse pré-natal na regulação da inflamação alérgica pulmonar no modelo murino de asma experimental 65 Iorque” para o estresse pós-natal. As fêmeas foram distribuídas em 4 grupos experimentais: CC: fêmeas não estressadas;CE: fêmeas estressadas pós-natalmente aos 60 dias de idade (PND60), EC: fêmeas nascidas de mães estressadas entre o dia 15 (GD15) e 18 de gestação (GD18); EE: fêmeas nascidas de mães estressadas entre o GD15 e GD18 e estressadas pós-natalmente aos PND60. A indução da inflamação alérgica pulmonar foi realizada através da sensibilização dos animais com solução de ovalbumina (OVA) 0,1 mg.Kg-1 sc para avaliação do leucograma, lavado broncoalveolar (BAL), celularidade hematopoiética medular e neuroquímica. Os experimentos foram realizados 24h após a última sessão de nebulização.O número de células do BAL foi significantemente maior nos animais do grupo EE, em relação àqueles dos grupos CC (P<0.01), CE (P<0.01) e EC (P<0.001). Na contagem diferencial do BAL os linfócitos e macrófagos do grupo EE foram significantemente maiores que em os outros grupos avaliados (P<0.05). Na contagem diferencial de células no sangue não foram observadas alterações (P>0.05) para os linfócitos, neutrófilos, eosinófilos e monócitos; porém, observou-se diferenças significativas (P<0.05) entre o número de bastonetes dos grupos, sendo maior nos animais do grupo CC em relação àquelesdo grupo EC. O número de células hematopoiéticas da medula óssea foi significantemente (P<0.05) menor nos animais do grupo EE, em relação àqueles do grupo CC. No córtex pré-frontal, há diferenças significantes na relação Ácido Homovanílico/Dopamina (HVA/DA) (P<0.05), sendo maior nos animais do grupo EC, em relação àqueles do grupo CE. Em conclusão,o estresse pré-natal levou a modulação de células do sistema imune (SI) dos neonatos, evidenciado após a exposição a estresse agudo pós-natal, amplificando a resposta alérgica pulmonar. Sugere-se que a maior susceptibilidade dos animais do grupo EE seja consequência de alterações induzidas pelo estresse pré-natal no eixo hipotálamo-pituitária-adrenal (HPA).spa
dc.description.abstractDue to the rapid growth of the fetus it is particularly vulnerable to insults and changes in hormonal milieu. Therefore, is suggested that adverse situations experienced by the pregnant mother can alter the development and health of offspring, mainly due to the permeability of the placental barrier to various hormones and substances. The aim of the present investigation was to study the effects of prenatal stress in the regulation of pulmonary allergic inflammation, employing the murine model of experimental asthma. For this purpose, were used virgin female mice, Swiss lineage, of 50 days old. The models used were foot shock to induce prenatally stress, and "New York subway" stress to induce postnatally stress. Females were divided into 4 groups: CC group: not stressed females; CE group: postnatally stressed females (PND60); EC: females born from stressed mothers (GD15 to GD18); EE Group: females born from stressed mothers (GD15 to GD18) (footshock) and postnatally stressed (PND60). The induction of allergic pulmonary inflammation was done through sensitization of animals with 0,1 mg.Kg-1 sc of ovalbumin (OVA) solution, to further evaluate leukogram, bronchoalveolar lavage (BAL) hematopoietic marrow cellularity and neurochemistry. The experiments were performed 24 hours after the last session of nebulization. The number of BAL cells was significantly higher in EE group animals compared with the CC group (P<0.01), CE (P<0.01) and CE (P<0.001). In the differential count of the BAL, lymphocytes and macrophages of EE group were significantly higher than the other groups studied (P<0.05). In the blood differential cell count were not observed changes (P>0.05) for lymphocytes, neutrophils, eosinophils and monocytes; however, there were significant differences (P<0.05) observed in the number of rods cells between groups, being higher in animals the CC group compared to EC group. The number of hematopoietic cells of the bone marrow was significantly lower (P<0.05) in animals of Group EE, compared with CC group. In the prefrontal cortex, there were significant differences in homovanillic acid /dopamine (HVA/DA) (P<0.05) rate, being higher in the EC group, compared to EC group. In conclusion, prenatal stress modulated the immune system (SI) cells of neonates, evidenced after exposure to a post-natal acute stress by amplification of pulmonary allergic response. It is suggested that the increased susceptibility of animals EE group is a result of changes induced by prenatal stress on hypothalamus pituitary-adrenal (HPA) axis.eng
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dc.identifier.doi10.22579/20112629.364
dc.identifier.eissn2011-2629
dc.identifier.issn0121-3709
dc.identifier.urihttps://repositorio.unillanos.edu.co/handle/001/2596
dc.identifier.urlhttps://doi.org/10.22579/20112629.364
dc.language.isospaspa
dc.publisherUniversidad de los Llanosspa
dc.relation.bitstreamhttps://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/364/pdf_53
dc.relation.bitstreamhttps://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/364/html_20
dc.relation.citationeditionNúm. 2 , Año 2016spa
dc.relation.citationendpage77
dc.relation.citationissue2spa
dc.relation.citationstartpage64
dc.relation.citationvolume20spa
dc.relation.ispartofjournalOrinoquiaspa
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dc.rightsOrinoquia - 2017spa
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dc.sourcehttps://orinoquia.unillanos.edu.co/index.php/orinoquia/article/view/364spa
dc.subjectaislamientospa
dc.subjectmicosisspa
dc.subjectpecesspa
dc.subjectsanidadspa
dc.subjectfisheseng
dc.subjectfungieng
dc.subjecthealtheng
dc.subjectisolationeng
dc.titleEfeito do estresse pré-natal na regulação da inflamação alérgica pulmonar no modelo murino de asma experimentalspa
dc.title.translatedEffect of prenatal stress in regulating pulmonary allergic inflammation in a murine model of experimental asthmaeng
dc.typeArtículo de revistaspa
dc.typeJournal Articleeng
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.type.coarversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
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dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.localSección Ciencias agrariasspa
dc.type.localSección Agricultural scienceseng
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
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