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Uso de fertilizante comercial en la cinética celular de Desmodesmus opoliensis (Chlorophyceae), reporte preliminar

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dc.contributor.authorBurgos Rada, Cristian A.spa
dc.contributor.authorRamírez-Merlano, Juan A.spa
dc.contributor.authorJiménez-Forero, Javier A.spa
dc.date.accessioned2016-12-16T00:00:00Z
dc.date.accessioned2024-07-25T18:14:38Z
dc.date.available2016-12-16T00:00:00Z
dc.date.available2024-07-25T18:14:38Z
dc.date.issued2016-12-16
dc.description.abstractLas microalgas son microorganismos fotosintéticos reconocidos por su producción de vitaminas, carbohidratos, pigmentos y lípidos. Sin embargo, esta producción es afectada por la composición de nutrientes micro y macrominerales en el sistema de cultivo, que para algunos casos constituyen altos costos, un 70% en la producción de microalgas. El objetivo de este estudio fue evaluar un fertilizante edáfico comercial como medio de cultivo para la microalga Desmodesmus opoliensis y su efecto en la cinética celular. Para esto, se utilizó como medio comercial Remital® en cultivos estáticos, evaluando un total de cuatro concentraciones (0.5; 1.0; 1.5 y 2.0 gr/l de agua destilada), este medio de cultivo fue comprando con el medio F/2 Guillard (1 ml/l) por triplicado para cada medio de cultivo (n=3). Para determinar la curva cinética y su comportamiento, la densidad celular (cel/ml) se llevó a cabo por medio de conteo celular en cámara de Neubauer y las clorofilas totales (μg/ml) por espectrofotometría, a una tempertura de cultivo de 242 °C y un fotoperiodo de 12:12 (Luz:Oscuridad) durante 14 días. El F/2 Guillard alcanzó una densidad celular máxima de 4.33±1.96 (106 cel/ml). El tratamiento con concentraciones de 2 g/l, mostró el mayor crecimiento con un promedio de 2.9x106 cel/m, sin diferencias estadísticas significativas al ser comparado con las diferentes concentraciones del fertilizante comercial Remital® (P>0.05). Estos resultados confirman y permiten el uso de fertilizantes no convencionales como el Remital® en la producción de Desmodesmus opolienis.spa
dc.description.abstractThe microalgae are photosynthetic microorganisms recognized for their production of vitamins, carbohydrates, pigments and lipids. However, this production is affected by the composition of micro and macro nutrients in the culture system,which in some cases constitute high costs, 70% in the production of microalgae. The objective of this study was to evaluate a commercial edaphic fertilizer as a culture medium for the microalgae Desmodesmus opoliensis and its effect on cell kinetics. For this, Remital® was used as a commercial medium in static cultures, evaluating a total of four concentrations (0.5, 1.0, 1.5 and 2.0 gr/l of distilled water), this culture medium was purchased with the Guillard F/2 medium (1 ml/l) in triplicate for each culture medium (n= 3). To determine the kinetic curve and its behavior, the cell density (cel/ml) was carried out by means of cell count in Neubauer chamber and total chlorophylls (μg/ml) by spectrophotometry, at a culture temperature of 24 ± 2 °C and a photoperiod of 12:12 (light:dark) for 14 days. The Guillard F/2 reached a maximum cell density of 4.33±1.96 (106 cells/ml). The treatment with concentrations of 2 g/l, showed the highest growth with an average of 2.9x106 cel/ml, without significant statistical differences when compared with the different concentrations of the commercial fertilizer Remital® (P>0.05). These results confirm and allow the use of unconventional fertilizers such as Remital® in the production of Desmodesmus opolienis.eng
dc.format.mimetypeapplication/pdfspa
dc.identifier.doi10.22579/20112629.438
dc.identifier.eissn2011-2629
dc.identifier.issn0121-3709
dc.identifier.urihttps://repositorio.unillanos.edu.co/handle/001/3910
dc.identifier.urlhttps://doi.org/10.22579/20112629.438
dc.language.isospaspa
dc.publisherUniversidad de los Llanosspa
dc.relation.bitstreamhttps://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/438/1028
dc.relation.citationendpage25
dc.relation.citationissue2 Supspa
dc.relation.citationstartpage18
dc.relation.citationvolume20spa
dc.relation.ispartofjournalOrinoquiaspa
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dc.rightsOrinoquia - 2019spa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.coarhttp://purl.org/coar/access_right/c_abf2spa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/spa
dc.sourcehttps://orinoquia.unillanos.edu.co/index.php/orinoquia/article/view/438spa
dc.subjectchlorophylleng
dc.subjectcell densityeng
dc.subjectedaphiceng
dc.subjectmicroalgaeeng
dc.subjectpigmentseng
dc.subjectclorofilaeng
dc.subjectdensidade celulareng
dc.subjectedáficaseng
dc.subjectmicroalgaseng
dc.subjectpigmentoseng
dc.subjectclorofilaspa
dc.subjectdensidad celularspa
dc.subjectedáficospa
dc.subjectmicroalgaspa
dc.subjectpigmentosspa
dc.titleUso de fertilizante comercial en la cinética celular de Desmodesmus opoliensis (Chlorophyceae), reporte preliminarspa
dc.title.translatedUse of commercial fertilizer in the cell kinetics of Desmodesmus opoliensis (Chlorophyceae), preliminary reporteng
dc.typeArtículo de revistaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.type.coarversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.localJournal articleeng
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dspace.entity.typePublicationspa

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