Publicación: Caracterización genética de Ichthyoelephas longirostris de los ríos La Miel y Ranchería usando marcadores microsatelites
dc.contributor.author | Perdomo-Aguirre, Yuly A. | spa |
dc.contributor.author | Cruz-Flor, Wilson R. | spa |
dc.contributor.author | Espinosa-León, Luisa P. | spa |
dc.contributor.author | Carrillo-Avila, Mauricio | spa |
dc.date.accessioned | 2014-09-01T00:00:00Z | |
dc.date.accessioned | 2024-07-25T18:14:26Z | |
dc.date.available | 2014-09-01T00:00:00Z | |
dc.date.available | 2024-07-25T18:14:26Z | |
dc.date.issued | 2014-09-01 | |
dc.description.abstract | El Ichthyoelephas longirostris comúnmente conocido como el Pataló es una especie endémica de la cuenca del río Magdalena y del río Ranchería. Posee una fuerte presión pesquera comercial y de consumo. Según el libro rojo de peces dulceacuícolas de Colombia actualmente se encuentra reportada como una especie amenazada debido a las fuertes alteraciones de su hábitat producidas principalmente por contaminación y erosión. Teniendo en cuenta su importancia y la falta de información genética reportada, en este estudio se analizaron seis marcadores microsatélites para determinar el grado de variabilidad y estructura genético-poblacional del Pataló de los ríos La Miel y Ranchería. Para las dos localidades la heterozigosidad esperada varió de 0,6182 a 0,546, la heterozigosidad observada entre 0,6812 a 0,454, la riqueza alélica entre 6,760 y 4,640 y la diversidad génica encontrada estuvo entre 0,631 y 0,540 respectivamente. El índice de endogamia FIS evidenció un exceso de heterocigotos dado por su valor medio negativo. Los análisis realizados indican que existen dos poblaciones diferentes de Pataló con una estructuración genética moderada, las cuales presentan un buen estado genético. Estos resultados son de gran valor por ser el primer estudio de caracterización genética realizado en esta especie y con el cual se podrán tomar medidas de control y manejo especialmente cuando ya se están implementando acciones de repoblamiento y se está trabajando en su reproducción en cautiverio. | spa |
dc.description.abstract | The Ichthyoelephas longirostris, commonly known as Pataló, is an endemic species from the Magdalena River and Rancheria River in Colombia. It is facing a b commercial and consumer fishing pressure. According to the red book of freshwater fishes of Colombia, it is identified as an endangered species due to b changes in its habitat, mainly caused by pollution and erosion. In this research, given its importance and the lack of genetic information reported, six microsatellite markers were analyzed to determine the degree of genetic variability and population structure of Pataló from La Miel and Rancheria rivers. For the two locations the expected heterozygosity ranged from 0.6182 to 0.546, the observed heterozygosity from 0.6812 to 0.454, allelic richness between 6.760 and 4.640, and the gene diversity ranged between 0.631 and 0.540, respectively. The Fis inbreeding index showed an excess of heterozygotes given its average negative value. The analysis howed two distinct populations of Pataló with moderate genetic structure, which exhibit a good genetic status. These results are of great value, given that this is the first study of genetic characterization conducted in this species. Based on these results control and management measures can be taken, relevant for repopulating actions being implemented with regard to reproduction in captivity. | eng |
dc.format.mimetype | application/pdf | spa |
dc.identifier.doi | 10.22579/20112629.368 | |
dc.identifier.eissn | 2011-2629 | |
dc.identifier.issn | 0121-3709 | |
dc.identifier.uri | https://repositorio.unillanos.edu.co/handle/001/3866 | |
dc.identifier.url | https://doi.org/10.22579/20112629.368 | |
dc.language.iso | spa | spa |
dc.publisher | Universidad de los Llanos | spa |
dc.relation.bitstream | https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/368/980 | |
dc.relation.citationedition | Núm. 2 , Año 2014 : Vol. 18 Núm 2 sup (2014) | spa |
dc.relation.citationendpage | 182 | |
dc.relation.citationissue | 2 | spa |
dc.relation.citationstartpage | 173 | |
dc.relation.citationvolume | 18 | spa |
dc.relation.ispartofjournal | Orinoquia | spa |
dc.relation.references | Arango L, Montes-R JM. Caracterización entomológica parcial de la cuenca del río La Miel en el departamento de Caldas (Colombia). Boletín científico centro de museos museo de historian. 2009;13(2):249-268. | spa |
dc.relation.references | Bárbara TC, Palma-Silva GM, Paggi F, Bered MF, Fay, Lexer C. Crossspecies transfer of nuclear microsatellite markers: potential and limitations. Mol Ecol. ;16(18):3759-3767.doi: 10.1111/j.1365294X.2007.03439.x [ Links ] Barroso RM, Hilsdorf AWS, Moreira HLM, Cabello PH, Traub-Cseko YM. Genetic diversity of wild and cultured populations of Brycon opalinus (Cuvier, 1819) (Characiforme, Characidae, Bryconiae) using microsatellites. Aquaculture. ;247:51-65. | spa |
dc.relation.references | Beltrán IC, Estrada M, Valderrama M. 2000. Plan de Ordenamiento: manejo y aprovechamiento sostenible pesquero y acuícola en la cuenca del Río Magdalena. Instituto Nacional de Pesca y Acuicultura- INPA. Santafé de Bogotá, D.C. | spa |
dc.relation.references | Benites C. 2008. Caracterização genética do pintado Pseudoplatystoma corruscans, (Spix & Agassiz, 1829) (Siluriformes: Pimelodidae) da Bacia Paraná-Paraguai, por marcadores moleculares do tipo microsatélite. Tese doutorado, Centro de Aquicultura da Universidade Estadual Paulista. | spa |
dc.relation.references | Castro MC, Vari R. Detritivores of the South American Fish Family Prochilodontidae (Teleostei: Ostariophysi: Characiformes): A Phylogenetic and Revisionary Study. Smith Contr Zool. 2004; 622:1-187 | spa |
dc.relation.references | Carvalho-Costa LF, Hatanaka T, Galetti PM. Isolation and characterization of polymorphic microsatellite markers in the migratory freshwater fish Prochilodus costatus. Mol Ecol Notes. 2006; 6(3):818-819. doi: 10.1111/j.1471-8286.2006.01356.x | spa |
dc.relation.references | Carvalho-Costa LF, Hatanaka T, Galetti PM. Evidence of lack of population substructuring in the Brazilian freshwater fish Prochilodus costatus. GenetiesMol Biol.2008; 31(Suppl 1):377380. | spa |
dc.relation.references | Cena CJ, Morgan GE, Malette MD, Heath DD. Inbreeding, outbreeding and environmental effects on genetic diversity in 46 walleye (Sander vitreus) populations. Mol Ecol. 2006; 15:303-320. | spa |
dc.relation.references | De Woody JA, Avise JC. Microsatellite variation in marine, freshwater and anadromous fishes compared with other animals. Journal do fish Biology. 2000; 56:461-473. | spa |
dc.relation.references | Espinosa-León LP, Carrillo-Ávila M. 2013. Estado de conservación de la población del bagre rayado Pseudoplatystoma magdaleniatum en la cuenca magdalénica, valoración de implementaciones ambientales y socioeconómicas y definición de escenarios de ordenación pesquera y sostenibilidad. Informe técnico, componente genético. | spa |
dc.relation.references | Fitzsimmons NN, Morits C, Moore SS. Conservation and dynamics of microsatellite loci over 300-million years of marine turtle evolution. Mol Biol Evol. 1995; 12:432-440. | spa |
dc.relation.references | Gaggiotti EO, Lange O, Rassman K, Gliddon C. A comparison of two indirect methods for estimating average levels of gene flow using microsatellite data. Mol Ecol. 1999; 8:1513-1520. | spa |
dc.relation.references | Goudet J. 2001. FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). Available from http://www.unil.ch/izea/softwares/fstat.html. Updated from Goudet (1995). | spa |
dc.relation.references | Hatanaka T, Galleti PM. RAPD marker indicate the occurrence of structured populations in a migratory fish species. Genet Mol Biol. 2003; 26(1):19-25. | spa |
dc.relation.references | Hatanaka T, Silva FH, Galetti PM. Population substructuring in a migratory freshwater fish Prochilodus argenteus (Characiformes, Prochilodontidae) from the São Francisco River. Genetic. 2006; 126:153-159. | spa |
dc.relation.references | Hilsdorf AW, Azeredo - Espin AM, Krieger MH, Krieger JE. Mitochondrial DNA diversity in wild and cultured populations of Brycon opalinus (curvier, 1819) (Characiformer, Characidae, Bryconinae) form the Paraiba do sul Basin, Brazil. Aquaculture. 2002; 214:81-91. | spa |
dc.relation.references | Kimura M, Crow F. The number of alleles that can be maintained in a finite population. Genetics. 1964; 49:725-738. | spa |
dc.relation.references | Laikre L, Palm S, Ryman N. Genetic population structure of fishes: implications for coastal zone management. Ambio. 2005; 34(2):111-119. | spa |
dc.relation.references | Mojica JI, Castellanos C, Usma S, Álvarez R. 2002. Libro rojo de peces dulceacuícolas de Colombia. La serie de Libros Rojos de Colombia. Instituto de Ciencias Naturales Universidad Nacional de Colombia, Ministerio del Medio Ambiente, Bogotá, Colombia. | spa |
dc.relation.references | Mojica J, Castellanos C, Sánchez P, Díaz C. Peces de la cuenca del rio ranchería, la guajira Colombia. Biota Colombia. ;7(1):129142. | spa |
dc.relation.references | Mojica JI, Castellanos C, Usma S, Álvarez R. 2012. Libro rojo de peces dulceacuícolas de Colombia. La serie de Libros Rojos de Colombia. Instituto de Ciencias Naturales Universidad Nacional de Colombia, Ministerio del Medio Ambiente, Bogotá, Colombia. | spa |
dc.relation.references | Nei M. 1987. Molecular Evolutionary Genetics. Columbia University Press, New York. | spa |
dc.relation.references | O´Connell M, Wright JM. Microsatellite DNA in fishes. Rev Fish Biol Fisher. 1997; 7:331-363. | spa |
dc.relation.references | Orozco-Berdugo G, Narváez- Barandica JC. Genetic diversity and population structure of bocachico Prochilodus magdalenea (Pisces, Prochilontidae) in the Magdalena River basin and its tributaries, Colombia. Genet Mol Biol. 2014; 37(1):37-45. | spa |
dc.relation.references | Patiño A. Cultivo experimental de peces en estanques. Cedespia. 1973; 2(5):75-128. | spa |
dc.relation.references | Petit RJ, El Mousadik A, Pons O. Identifying Populations For Conservation On The Basis Of Genetic Markers. Conserv Biol. 1998; 12:844-855. | spa |
dc.relation.references | Polania J, Orosco C, Ángel L. Delta del rio ranchería (la guaria, Colombia): caudal salinidad y transporte de sólidos y sus posibles influencias sobre la composición y estructura de los manglares. Actual Biol. 2006; 28(84):27-37 | spa |
dc.relation.references | Povh JA, Lopera Barrero NM, Ribeiro RP, Lupchinski E, Gomes PC, López TS. Monitoreo genético en programas de repoblamiento de peces mediante marcadores moleculares. Cien Inv Agr. 2008; 35(1):5-15. | spa |
dc.relation.references | Raymond M, Rousset. GENEPOP (version 1.2): A population genetic software for exact test and ecumenism. J Hered. 1995; 86:248249. | spa |
dc.relation.references | Revaldaves E, Pereira LH, Foresti F, Oliveira C. Isolation and characterization of microsatellite loci in Pseudoplatystoma corruscans (Siluriformes: Pimelodidae) and cross- species amplification. Mol Ecol Notes. 2005; 5:463-465. DOI:10.1111/ j.14718286.2005.00883x | spa |
dc.relation.references | Rice WR. Analyzing tables of statistical tests. Evolution. 1989; 43:223-225. | spa |
dc.relation.references | Rico C, Rico I, Hewitt G. 470 millions years of conservation of microsa-tellite loci among fish species. Proc R Soc Lond B. 1996; 263:549-557. | spa |
dc.relation.references | Román C. 1993. Historia natural del jetudo, Ichthyoelephas longirostris (Steindachner 1989) (Pisces: prochilodontidae) en la cuenca del rio la vieja, alto Magdalena, Colombia. | spa |
dc.relation.references | Roman-Valencia C, Ortiz-Muñoz V. Sobre la reproducción de Ichthyoelephas longirostris (Pisces, Prochilodontidae) en la cuenca del Rio Magdalena, Colombia. Dahlia Revista de Asociacion Colombiana de Ictiologia. 2001; 4:33-35. | spa |
dc.relation.references | Rueda E, Sommer J, Scarabotti P, Markariani R, Ortí G. Isolation and characterization of polymorphic microsatellite loci in the migratory freshwater fish Prochilodus lineatus (Characiformes, Prochilodontidae). Conserv Genet Resource. 2011; 3:681-684. | spa |
dc.relation.references | Sánchez A, Galetti PM. Microsatellite Loci isolated in the Freswater fish Brycon hilarii. Mol Ecol Notes. 2006; 6:1045-1046. | spa |
dc.relation.references | Sánchez A. 2007. Estructura genética poblacional de Brycon hilarii (characidae) da sub-bacia do Rio Miranda, e seu significado para programas de conservação. Tese Doutorado. Universidade Federal de São Carlos. | spa |
dc.relation.references | Saura M, Caballero P, Caballero A, Morán P. Genetic variation in restored Atlantic salmon (Salmo salar L.) populations in the Ulla and Lérez rivers, Galicia, Spain. ICES Journal of Marine Science. 2006; 63:1290-1296. | spa |
dc.relation.references | Schlötterer C, B. Amos y D. Tautz. (1991). Conservation of polymorphic simple sequence loci in cetacean species. Nature, 354:6365. | spa |
dc.relation.references | Schlötterer C, Amos B, Tautz D. 1992. Slippage synthesis of simple sequence DNA. Nuclei Acids Research, 20: 211-215. | spa |
dc.relation.references | Wang S, Hard JJ, Utter F. 2002. Salmonid inbreeding: a review. Reviews in Fisheries Biology and Fisheries, 11:301-319. | spa |
dc.relation.references | Wasko AP, Galetti PM. 2002 RAPD analysis in the Neotropical fish Brycon lundii: Genetic diversity and its implications for the conservation of the species. Hydrobiology, 474:131-137. | spa |
dc.relation.references | Weir BS, Cockerham CC. 1984. Estimating F-statistics for the analysis of population structure. Evolution, 38: 1358-1370. | spa |
dc.relation.references | Wright S.1978. Variability within and among natural populations. v.4. The University of Chicago Press. | spa |
dc.relation.references | Zane LL, Bargelloni, Patarnello T. 2002. Strategies for microsatellite isolation: a review. Molecular Ecology, 11: 1-16. | spa |
dc.rights | Orinoquia - 2017 | spa |
dc.rights.accessrights | info:eu-repo/semantics/openAccess | spa |
dc.rights.coar | http://purl.org/coar/access_right/c_abf2 | spa |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-sa/4.0/ | spa |
dc.source | https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/view/368 | spa |
dc.subject | Peces de agua dulce | spa |
dc.subject | estructura poblacional | spa |
dc.subject | conservación | spa |
dc.subject | variabilidad genética | spa |
dc.subject | Freshwater fish | eng |
dc.subject | population structure | eng |
dc.subject | conservation | eng |
dc.subject | genetic variability | eng |
dc.title | Caracterización genética de Ichthyoelephas longirostris de los ríos La Miel y Ranchería usando marcadores microsatelites | spa |
dc.title.translated | Genetic characterization of Ichthyoelephas longirostris La Miel and Ranchería Rivers using markers microsatellites | eng |
dc.type | Artículo de revista | spa |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | spa |
dc.type.coarversion | http://purl.org/coar/version/c_970fb48d4fbd8a85 | spa |
dc.type.content | Text | spa |
dc.type.driver | info:eu-repo/semantics/article | spa |
dc.type.local | Journal article | eng |
dc.type.version | info:eu-repo/semantics/publishedVersion | spa |
dspace.entity.type | Publication | spa |