Publicación: Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando
dc.contributor.author | Vejarano-Orjuela, Álvaro | spa |
dc.contributor.author | Hernández-Vásquez, Aureliano | spa |
dc.contributor.author | Rondón-Barragán, Iang S. | spa |
dc.contributor.author | Suárez-Mesa, Rafael A. | spa |
dc.date.accessioned | 2020-05-11 00:00:00 | |
dc.date.accessioned | 2022-06-13T17:42:37Z | |
dc.date.available | 2020-05-11 00:00:00 | |
dc.date.available | 2022-06-13T17:42:37Z | |
dc.date.issued | 2020-05-11 | |
dc.description.abstract | El gen del receptor para la hormona luteinizante (LHR-LHCGR) es un gen altamente conservado y su mRNA origina diferentes variantes por corte y empalme alternativo (splicing alternativo). En bovinos se han reportado variantes que traducen en proteína pero ésta no atraviesa la membrana celular; en humanos ante una deleción del exón 10 no hay respuesta a la acción de la LH, por lo tanto, es razonable asumir que las diferencias en la respuesta a la LH estén relacionadas con la expresión de los LHR. El objetivo de este trabajo fue comparar entre la raza criolla Colombiana Romosinuano y la raza Brahman los posibles polimorfismos del ARNm del gen como potenciales indicadores de fertilidad. Se tomaron 5 vacas ciclando de cada raza se extirpó el ovario que contenía un folículo preovulatorio, se retiró la pared folicular, se extrajo el RNA total y se sintetizó, amplificó, secuenció y comparó el ADNc contra secuencias ya reportadas, presentando una alta similitud. Se concluye que en estas razas la ausencia de variantes en la expresión del gen, estaría asociada a buen desempeño reproductivo. | spa |
dc.description.abstract | The luteinizing hormone (LH) choriogonadotropin receptor (LHCGR) gene is highly conserved and its mRNA causes variants by alternative splicing. Variants have been reported in cattle that translate into protein; however, this does not cross the cell membrane. Exon 10 deletion in humans does not trigger a response to the action of LH; it is therefore reasonable to assume that differences regarding response to LH are related to LHR expression. This work was aimed at comparing the Colombian creole Romosinuano breed to the Brahman breed regarding possible HCGR gene mRNA polymorphisms as potential fertility indicators. Five cycling cows were taken from each breed and the ovaries containing pre-ovulatory follicles were excised, the follicular wall was removed and total RNA extracted. The cDNA was synthesised, amplified, sequenced and compared to already reported sequences, great similarity being observed. It was concluded that the lack of variants regarding gene expression in these breeds would be associated with good reproductive performance. | eng |
dc.format.mimetype | application/pdf | spa |
dc.identifier.doi | 10.22579/20112629.590 | |
dc.identifier.eissn | 2011-2629 | |
dc.identifier.issn | 0121-3709 | |
dc.identifier.uri | https://repositorio.unillanos.edu.co/handle/001/2743 | |
dc.identifier.url | https://doi.org/10.22579/20112629.590 | |
dc.language.iso | spa | spa |
dc.publisher | Universidad de los Llanos | spa |
dc.relation.bitstream | https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/590/pdf | |
dc.relation.citationedition | Núm. 1 , Año 2020 | spa |
dc.relation.citationendpage | 51 | |
dc.relation.citationissue | 1 | spa |
dc.relation.citationstartpage | 42 | |
dc.relation.citationvolume | 24 | spa |
dc.relation.ispartofjournal | Orinoquia | spa |
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dc.rights | Orinoquia - 2020 | spa |
dc.rights.accessrights | info:eu-repo/semantics/openAccess | spa |
dc.rights.coar | http://purl.org/coar/access_right/c_abf2 | spa |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | spa |
dc.source | https://orinoquia.unillanos.edu.co/index.php/orinoquia/article/view/590 | spa |
dc.subject | Inbreeding | eng |
dc.subject | heterosis | eng |
dc.subject | crossing | eng |
dc.subject | additive | eng |
dc.subject | dominant | eng |
dc.subject | combining ability | eng |
dc.subject | Endogamia | spa |
dc.subject | heterosis | spa |
dc.subject | cruzamiento | spa |
dc.subject | aditivo | spa |
dc.subject | dominante | spa |
dc.subject | habilidad combinatoria | spa |
dc.title | Polimorfismos del gen del receptor para la hormona luteinizante (LHR – LHGCR) en folículos ováricos de vacas Romosinuano y Brahman ciclando | spa |
dc.title.translated | Luteinizing hormone choriogonadotropin receptor (LHGCR) gene polymorphism in ovarian follicles from cycling Romosinuano and Brahman cows | eng |
dc.type | Artículo de revista | spa |
dc.type | Journal Article | eng |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | spa |
dc.type.coarversion | http://purl.org/coar/version/c_970fb48d4fbd8a85 | spa |
dc.type.content | Text | spa |
dc.type.driver | info:eu-repo/semantics/article | spa |
dc.type.local | Sección Ciencias agrarias | spa |
dc.type.local | Sección Agricultural sciences | eng |
dc.type.version | info:eu-repo/semantics/publishedVersion | spa |
dspace.entity.type | Publication |