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MONCALEANO, J.S. | 2007-01-01
Titulo en ingles: Mosaicism leucocitary associated to infertility in four mares.RESUMEN: Se tomaron muestras de sangre periférica a cuatro yeguas de 19, 13, 10 y 4 años de edad, de raza Silla francesa, dos Criollas Colombianas y una Paso Fino Colombiano respectivamente, para realizar cultivo de linfocitos. Los animales fueron remitidos por la Clínica de Reproducción al Laboratorio de Citogenética Animal de la Facultad de Medicina Veterinaria y de Zootecnia de la Universidad Nacional de Colombia por antecedentes de problemas reproductivos. A la palpación se detectaron órganos reproductivos subdesarrollados; ausencia de preñez, además abortos frecuentes y en algunos casos nacimiento de potros inmaduros o con defectos fenotípicos. La evaluación citogenética se realizó mediante análisis de cromosomas obtenidos a partir de cultivo de linfocitos, y teñidos con Giemsa. El complemento cromosómico en todos los animales estudiados fue 63, XO/64,XX; las células con monosomía del X, (63,XO) oscilaron entre el 25% y 63% de las células analizadas y las células normales (64,XX) entre el 37% y el 75% . Los resultados de estos análisis demostraron los beneficios de este tipo de diagnostico para determinar fallas reproductivas que involucran anormalidades cromosómicas en yeguas que fenotipicamen no presentaban alteraciones evidentes.Palabras clave: monosomía, cromosomas sexuales, mosaicismo en yeguas.ABSTRACT: Heparinized blood samples for culturing leucocytes were taken from four mares that were 4, 10, 13, and 19 years old. They were of three different breeds. One was a French saddle horse, two were Colombian creoles, and the fourth was a fine stepping Colombian riding horse. The mares were remitted by the Clinic of Reproduction to the Animal Cytogenetic Laboratory of the School of Veterinary Medicine and Zootechnics of the National University of Colombia due to their history of reproductive failure. Rectal palpation revealed atrophic reproductive organs. Failure to conceive, frequent abortions, and birth of inmature foals, or those born with phenotypic defects, were noted in the mares’ histories. A cytogenetic evaluation was done by an analysis of the mares’ chromosomes that were obtained by culturing the leucocytes and staining them with Giemsa. The chromosome complement was 63,XO/64,XX for all samples. The cells analyzed with X monosomy (63,XO) varied from 25% to 63%, and the normal cells (64,XX) varied from 37% to 75%. The results of this analysis demonstrated that this diagnostic tool is beneficial to determine reproductive failures due to chromosomal abnormalities in mares that didn’t show any evident phenotypic abnormalities.Key work: monosomy, sexual chromosome, mare mosaicism.
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