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Preliminary results of in vitro fertilization for the endangered murciano-levantina bovine breed

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dc.contributor.authorBarajas, DPspa
dc.contributor.authorVelásquez, JGspa
dc.contributor.authorand Coy P, Poto Aspa
dc.date.accessioned2004-01-01 00:00:00
dc.date.accessioned2022-06-13T17:40:57Z
dc.date.available2004-01-01 00:00:00
dc.date.available2022-06-13T17:40:57Z
dc.date.issued2004-01-01
dc.description.abstractASBTRACT: The Murciano-levantina  cattle from the Spanish South-East belongs to a  bovine endangered breed under the special protection of FAO (WWL-DAD:3)*. Their characteristics of rusticity,  longe- vity, docility and disease resistance make interesting the recovery of the breed. The objective of the present work   was   to   deter mine   the efficiency of in vitro bovine embryo production using oocytes collected from post mortem ovaries fertilized with frozen thaw sperm obtained either from commercial or Murciano-levantina bulls. Cumulus oocyte complexes were matured for 24 h in TCM199 with 10% FBS, 2mM L-glutamine, 0.2 mM sodium pyruvate, 0.3  µg/mL LH and µg/mL FSH. Oocytes presumed matured  were fertilized with   Percoll-prepared   sper m (750.000/mL) in IVF-TALP as described by Parrish  et al. (Biol. Reprod  38:1171-1180, 1988). Zygotes (at 18 h) or two-cell stage embryos (at  48  h) were assessed by staining  with Hoechst 33342 (µg/mL; 20 min) under an epifluorescence microscopy. The experiment  was replicated  on 4 occasions.The results  of penetration  (PEN-%), average number of spermato- zoa inside the oocyte (S/O), monos permy (MON- %) putative embryos  (zygotes with two pronuclei, 2 PN-%) and percentages of cleavage at 48 hours (Table 1) were recorded. The results show that early embryos can be obtained with no difference with the source of sperm used in the monospermy and the cleavage rate between commercial and Mur- ciano-levantina  sperm (p>0.05). *Lista Mundial de Vigilancia para la Diversidad de los Animales Do- mésticos (1997). http:// www.fao.org/docrep/V8300S/ V8300S00.htmspa
dc.description.abstractASBTRACT: The Murciano-levantina  cattle from the Spanish South-East belongs to a  bovine endangered breed under the special protection of FAO (WWL-DAD:3)*. Their characteristics of rusticity,  longe- vity, docility and disease resistance make interesting the recovery of the breed. The objective of the present work   was   to   deter mine   the efficiency of in vitro bovine embryo production using oocytes collected from post mortem ovaries fertilized with frozen thaw sperm obtained either from commercial or Murciano-levantina bulls. Cumulus oocyte complexes were matured for 24 h in TCM199 with 10% FBS, 2mM L-glutamine, 0.2 mM sodium pyruvate, 0.3  µg/mL LH and µg/mL FSH. Oocytes presumed matured  were fertilized with   Percoll-prepared   sper m (750.000/mL) in IVF-TALP as described by Parrish  et al. (Biol. Reprod  38:1171-1180, 1988). Zygotes (at 18 h) or two-cell stage embryos (at  48  h) were assessed by staining  with Hoechst 33342 (µg/mL; 20 min) under an epifluorescence microscopy. The experiment  was replicated  on 4 occasions.The results  of penetration  (PEN-%), average number of spermato- zoa inside the oocyte (S/O), monos permy (MON- %) putative embryos  (zygotes with two pronuclei, 2 PN-%) and percentages of cleavage at 48 hours (Table 1) were recorded. The results show that early embryos can be obtained with no difference with the source of sperm used in the monospermy and the cleavage rate between commercial and Mur- ciano-levantina  sperm (p>0.05). *Lista Mundial de Vigilancia para la Diversidad de los Animales Do- mésticos (1997). http:// www.fao.org/docrep/V8300S/ V8300S00.htmeng
dc.format.mimetypeapplication/mswordspa
dc.format.mimetypeapplication/pdfspa
dc.format.mimetypetext/htmlspa
dc.identifier.doi10.22579/20112629.186
dc.identifier.eissn2011-2629
dc.identifier.issn0121-3709
dc.identifier.urihttps://repositorio.unillanos.edu.co/handle/001/2423
dc.identifier.urlhttps://doi.org/10.22579/20112629.186
dc.language.isospaspa
dc.publisherUniversidad de los Llanosspa
dc.relation.bitstreamhttps://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/186/341
dc.relation.bitstreamhttps://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/186/342
dc.relation.bitstreamhttps://orinoquia.unillanos.edu.co/index.php/orinoquia/article/download/186/643
dc.relation.citationeditionNúm. 1 , Año 2004spa
dc.relation.citationendpage25
dc.relation.citationissue1spa
dc.relation.citationstartpage25
dc.relation.citationvolume8spa
dc.relation.ispartofjournalOrinoquiaspa
dc.rightsOrinoquia - 2014spa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.coarhttp://purl.org/coar/access_right/c_abf2spa
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/spa
dc.sourcehttps://orinoquia.unillanos.edu.co/index.php/orinoquia/article/view/186spa
dc.subjectDenguespa
dc.subjectdengue hemorrágicospa
dc.subjectepidemiologíaspa
dc.subjectenfermedades tropicalesspa
dc.subjectdengue hemorrhagicspa
dc.subjectepidemiologyspa
dc.subjecttropical diseasesspa
dc.titlePreliminary results of in vitro fertilization for the endangered murciano-levantina bovine breedspa
dc.title.translatedPreliminary results of in vitro fertilization for the endangered murciano-levantina bovine breedeng
dc.typeArtículo de revistaspa
dc.typeJournal Articleeng
dc.type.coarhttp://purl.org/coar/resource_type/c_6501spa
dc.type.coarversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.localSección Artículosspa
dc.type.localSección Articleseng
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dspace.entity.typePublication

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